biostudies-literatureDe Vega JEarlham Institute (Funder)Biotechnology and Biological Sciences Research CouncilEngineering and Physical Sciences Research Council29https://www.ebi.ac.uk/biostudies/studies/S-EPMC7921889biostudies-literatureUnknown10<i>Miscanthus sacchariflorus</i> (Maxim.) Hack. is a highly productive C4 perennial rhizomatous biofuel grass crop. <i>M. sacchariflorus</i> is among the most widely distributed species in the genus, particularly at cold northern latitudes, and is one of the progenitor species of the commercial <i>M. × giganteus</i> genotypes. We generated a 2.54 Gb whole-genome assembly of the diploid <i>M. sacchariflorus</i> cv. "Robustus 297" genotype, which represented ~59% of the expected total genome size. We later anchored this assembly using the chromosomes from the <i>M. sinensis</i> genome to generate a second assembly with improved contiguity. We annotated 86,767 and 69,049 protein-coding genes in the unanchored and anchored assemblies, respectively. We estimated our assemblies included ~85% of F1000ResearchDraft genome assembly of the biofuel grass crop <i>Miscanthus sacchariflorus</i>.PMC7921889EP/S000771/1BBS/E/T/000PR9814BBS/E/W/10963A01ABBS/E/T/000PR9818BB/M029271/1BBS/E/W/0012843AEI-G-JdVDyer SDe Vega JFarrar KDonnison IfalseDraft genome assembly of the biofuel grass crop <i>Miscanthus sacchariflorus</i>.<i>Miscanthus sacchariflorus</i> (Maxim.) Hack. is a highly productive C4 perennial rhizomatous biofuel grass crop. <i>M. sacchariflorus</i> is among the most widely distributed species in the genus, particularly at cold northern latitudes, and is one of the progenitor species of the commercial <i>M. × giganteus</i> genotypes. We generated a 2.54 Gb whole-genome assembly of the diploid <i>M. sacchariflorus</i> cv. "Robustus 297" genotype, which represented ~59% of the expected total genome size. We later anchored this assembly using the chromosomes from the <i>M. sinensis</i> genome to generate a second assembly with improved contiguity. We annotated 86,767 and 69,049 protein-coding genes in the unanchored and anchored assemblies, respectively. We estimated our assemblies included ~85% of 2021-01-01T00:00:00Z20212025-04-03T21:33:41.231Z2025-04-03T21:33:41.231ZS-EPMC79218893373243310.12688/f1000research.44714.1