{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Han Z"],"funding":["Natural Science Foundation of Shanghai"],"pagination":["142"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7923655"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(1)"],"pubmed_abstract":["<h4>Background</h4>Invasive malignant pleomorphic adenoma (IMPA) is a highly invasive parotid gland tumor and lacks effective therapy. N6-Methyladenosine (m<sup>6</sup>A) is the most prevalent post-transcriptional modification of mRNAs in eukaryotes and plays an important role in the pathogenesis of multiple tumors. However, the significance of m<sup>6</sup>A-modified mRNAs in IMPA has not been elucidated to date. Hence, in this study, we attempted to profile the effect of IMPA in terms of m<sup>6</sup>A methylation in mRNA.<h4>Methods</h4>Methylated RNA immunoprecipitation with next-generation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) were utilized to acquire the first transcriptome-wide profiling of the m<sup>6</sup>A methylome map in IMPA followed by bioinformatics analysis.<h"],"journal":["Cancer cell international"],"pubmed_title":["Comprehensive analysis of the transcriptome-wide m<sup>6</sup>A methylome in invasive malignant pleomorphic adenoma."],"pmcid":["PMC7923655"],"funding_grant_id":["18ZR1422200"],"pubmed_authors":["Tian Z","Wang Q","Hu Y","Yang B","Han Z","Wu Y"],"additional_accession":[]},"is_claimable":false,"name":"Comprehensive analysis of the transcriptome-wide m<sup>6</sup>A methylome in invasive malignant pleomorphic adenoma.","description":"<h4>Background</h4>Invasive malignant pleomorphic adenoma (IMPA) is a highly invasive parotid gland tumor and lacks effective therapy. N6-Methyladenosine (m<sup>6</sup>A) is the most prevalent post-transcriptional modification of mRNAs in eukaryotes and plays an important role in the pathogenesis of multiple tumors. However, the significance of m<sup>6</sup>A-modified mRNAs in IMPA has not been elucidated to date. Hence, in this study, we attempted to profile the effect of IMPA in terms of m<sup>6</sup>A methylation in mRNA.<h4>Methods</h4>Methylated RNA immunoprecipitation with next-generation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) were utilized to acquire the first transcriptome-wide profiling of the m<sup>6</sup>A methylome map in IMPA followed by bioinformatics analysis.<h","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2025-04-19T06:33:21.711Z","creation":"2025-04-19T06:33:21.711Z"},"accession":"S-EPMC7923655","cross_references":{"pubmed":["33653351"],"doi":["10.1186/s12935-021-01839-6"]}}