biostudies-literatureHan ZNatural Science Foundation of Shanghai142https://www.ebi.ac.uk/biostudies/studies/S-EPMC7923655biostudies-literatureUnknown21(1)<h4>Background</h4>Invasive malignant pleomorphic adenoma (IMPA) is a highly invasive parotid gland tumor and lacks effective therapy. N6-Methyladenosine (m⁶A) is the most prevalent post-transcriptional modification of mRNAs in eukaryotes and plays an important role in the pathogenesis of multiple tumors. However, the significance of m⁶A-modified mRNAs in IMPA has not been elucidated to date. Hence, in this study, we attempted to profile the effect of IMPA in terms of m⁶A methylation in mRNA.<h4>Methods</h4>Methylated RNA immunoprecipitation with next-generation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) were utilized to acquire the first transcriptome-wide profiling of the m⁶A methylome map in IMPA followed by bioinformatics analysis.<hCancer cell internationalComprehensive analysis of the transcriptome-wide m⁶A methylome in invasive malignant pleomorphic adenoma.PMC792365518ZR1422200Tian ZWang QHu YYang BHan ZWu YfalseComprehensive analysis of the transcriptome-wide m⁶A methylome in invasive malignant pleomorphic adenoma.<h4>Background</h4>Invasive malignant pleomorphic adenoma (IMPA) is a highly invasive parotid gland tumor and lacks effective therapy. N6-Methyladenosine (m⁶A) is the most prevalent post-transcriptional modification of mRNAs in eukaryotes and plays an important role in the pathogenesis of multiple tumors. However, the significance of m⁶A-modified mRNAs in IMPA has not been elucidated to date. Hence, in this study, we attempted to profile the effect of IMPA in terms of m⁶A methylation in mRNA.<h4>Methods</h4>Methylated RNA immunoprecipitation with next-generation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) were utilized to acquire the first transcriptome-wide profiling of the m⁶A methylome map in IMPA followed by bioinformatics analysis.<h2021-01-01T00:00:00Z2021 Mar2025-04-19T06:33:21.711Z2025-04-19T06:33:21.711ZS-EPMC79236553365335110.1186/s12935-021-01839-6