{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lemos D"],"funding":["HHS | National Institutes of Health","Governo Brasil","NCATS NIH HHS","ACL HHS","NIAID NIH HHS","NHLBI NIH HHS","HHS | Centers for Disease Control and Prevention","NIH HHS","NCEZID CDC HHS"],"pagination":["e01605-20"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7925200"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["94(24)"],"pubmed_abstract":["Although fetal death is now understood to be a severe outcome of congenital Zika syndrome, the role of viral genetics is still unclear. We sequenced Zika virus (ZIKV) from a rhesus macaque fetus that died after inoculation and identified a single intrahost substitution, M1404I, in the ZIKV polyprotein, located in nonstructural protein 2B (NS2B). Targeted sequencing flanking position 1404 in 9 additional macaque mothers and their fetuses identified M1404I at a subconsensus frequency in the majority (5 of 9, 56%) of animals and some of their fetuses. Despite its repeated presence in pregnant macaques, M1404I has occurred rarely in humans since 2015. Since the primary ZIKV transmission cycle is human-mosquito-human, mutations in one host must be retained in the alternate host to be perpetuate"],"journal":["Journal of virology"],"pubmed_title":["Two Sides of a Coin: a Zika Virus Mutation Selected in Pregnant Rhesus Macaques Promotes Fetal Infection in Mice but at a Cost of Reduced Fitness in Nonpregnant Macaques and Diminished Transmissibility by Vectors."],"pmcid":["PMC7925200"],"funding_grant_id":["1R01HL105704","U01 CK000516","1U01CK000516","UC Davis School of Veterinary Medicine Graduate Student Support Program","UL1 TR001863","Science without Borders","R21 AI129479","1R33AI129077","1R21AI129479-S","P51OD011107","R01 HL105704","Division of Intramural Research Program","R43 AI129077","U01CK000516","UC Davis","P51 OD011107"],"pubmed_authors":["Louie W","Saraf S","Pletnev AG","Borucki M","Singapuri A","Tsetsarkin KA","Van Rompay KKA","Usachenko J","Keesler RI","Grubaugh ND","Andersen KG","Chiu CY","Sanchez-San Martin C","Martyn C","Watanabe J","Li T","Allen J","Thissen J","Stuart JB","Lemos D","Coffey LL","Ramirez AL","Oliveira G"],"additional_accession":[]},"is_claimable":false,"name":"Two Sides of a Coin: a Zika Virus Mutation Selected in Pregnant Rhesus Macaques Promotes Fetal Infection in Mice but at a Cost of Reduced Fitness in Nonpregnant Macaques and Diminished Transmissibility by Vectors.","description":"Although fetal death is now understood to be a severe outcome of congenital Zika syndrome, the role of viral genetics is still unclear. We sequenced Zika virus (ZIKV) from a rhesus macaque fetus that died after inoculation and identified a single intrahost substitution, M1404I, in the ZIKV polyprotein, located in nonstructural protein 2B (NS2B). Targeted sequencing flanking position 1404 in 9 additional macaque mothers and their fetuses identified M1404I at a subconsensus frequency in the majority (5 of 9, 56%) of animals and some of their fetuses. Despite its repeated presence in pregnant macaques, M1404I has occurred rarely in humans since 2015. Since the primary ZIKV transmission cycle is human-mosquito-human, mutations in one host must be retained in the alternate host to be perpetuate","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Nov","modification":"2026-05-03T08:42:02.614Z","creation":"2021-03-13T08:17:01Z"},"accession":"S-EPMC7925200","cross_references":{"pubmed":["32999034"],"doi":["10.1128/JVI.01605-20"]}}