biostudies-literatureZang YYouth project of Shanghai Municipal Commission of Health and Family PlanningPlanned Science Program of the Shanghai University of Traditional Chinese MedicineScience and Technology Innovation Project of Putuo District Health SystemExploration Program of Putuo HospitalNational Natural Science Foundation of ChinaKey Medical Discipline Project of Shanghai Municipal Health Bureau1453-1464https://www.ebi.ac.uk/biostudies/studies/S-EPMC7925475biostudies-literatureUnknown48(2)Loss of podocytes is a hallmark of diabetic nephropathy, and a growing body of evidence indicates that podocytes are susceptible to palmitic acid (PA). We have previously shown that AS-IV inhibited PA-induced podocyte apoptosis by activating sarcoendoplasmic reticulum Ca²⁺ ATPase (SERCA), which indicate calcium regulation may involve in the process. Immunofluorescence staining, Western blot and flow cytometry were used to measure the protective efficacy of AS-IV to ameliorate PA-induced ER stress and podocyte apoptosis. Meanwhile, AS-IV inhibited cytochrome c release, decreased mitochondrial membrane potential, accompany with the depletion of endoplasmic reticulum Ca²⁺ and elevation of cytosolic and mitochondrial Ca²⁺. Sequestration of cytosolic calcium witMolecular biology reportsAstragaloside IV inhibits palmitic acid-induced apoptosis through regulation of calcium homeostasis in mice podocytes.PMC792547520154Y013381403235, 81803921PTKWWS2020012020360A2020LK070ZK2019A12Shan XLi ZPeng WZang YLiu SWang HWang YDeng WCao AWang LfalseAstragaloside IV inhibits palmitic acid-induced apoptosis through regulation of calcium homeostasis in mice podocytes.Loss of podocytes is a hallmark of diabetic nephropathy, and a growing body of evidence indicates that podocytes are susceptible to palmitic acid (PA). We have previously shown that AS-IV inhibited PA-induced podocyte apoptosis by activating sarcoendoplasmic reticulum Ca²⁺ ATPase (SERCA), which indicate calcium regulation may involve in the process. Immunofluorescence staining, Western blot and flow cytometry were used to measure the protective efficacy of AS-IV to ameliorate PA-induced ER stress and podocyte apoptosis. Meanwhile, AS-IV inhibited cytochrome c release, decreased mitochondrial membrane potential, accompany with the depletion of endoplasmic reticulum Ca²⁺ and elevation of cytosolic and mitochondrial Ca²⁺. Sequestration of cytosolic calcium wit2021-01-01T00:00:00Z2021 Feb2025-04-21T16:58:55.743Z2025-04-21T16:58:55.743ZS-EPMC79254753360615110.1007/s11033-021-06204-4