biostudies-literatureMcCann JRAmerican Heart AssociationNIDDK NIH HHSNational Institute of Diabetes and Digestive and Kidney Diseases569-578https://www.ebi.ac.uk/biostudies/studies/S-EPMC7927749biostudies-literatureUnknown29(3)<h4>Objective</h4>The purpose of this study was to establish a biorepository of clinical, metabolomic, and microbiome samples from adolescents with obesity as they undergo lifestyle modification.<h4>Methods</h4>A total of 223 adolescents aged 10 to 18 years with BMI ≥95th percentile were enrolled, along with 71 healthy weight participants. Clinical data, fasting serum, and fecal samples were collected at repeated intervals over 6 months. Herein, the study design, data collection methods, and interim analysis-including targeted serum metabolite measurements and fecal 16S ribosomal RNA gene amplicon sequencing among adolescents with obesity (n = 27) and healthy weight controls (n = 27)-are presented.<h4>Results</h4>Adolescents with obesity have higher serum alanine aminotransferase, C-reactiObesity (Silver Spring, Md.)The Pediatric Obesity Microbiome and Metabolism Study (POMMS): Methods, Baseline Data, and Early Insights.PMC7927749K23 DK120960R24 DK110492R24‐DK11049217SFRN33670990Catherine CWootton JRoche KArlotto MMcCann JRGranek JAWei ZSilverman JJawahar JIlkayeva OZizzi AArmstrong SCSarria CSeed PCNewgard CBDavid LAPoppe LKwee LCDressman HKBihlmeyer NAValdivia RHAnderson PRawls JFShah SHYounge NEfalseThe Pediatric Obesity Microbiome and Metabolism Study (POMMS): Methods, Baseline Data, and Early Insights.<h4>Objective</h4>The purpose of this study was to establish a biorepository of clinical, metabolomic, and microbiome samples from adolescents with obesity as they undergo lifestyle modification.<h4>Methods</h4>A total of 223 adolescents aged 10 to 18 years with BMI ≥95th percentile were enrolled, along with 71 healthy weight participants. Clinical data, fasting serum, and fecal samples were collected at repeated intervals over 6 months. Herein, the study design, data collection methods, and interim analysis-including targeted serum metabolite measurements and fecal 16S ribosomal RNA gene amplicon sequencing among adolescents with obesity (n = 27) and healthy weight controls (n = 27)-are presented.<h4>Results</h4>Adolescents with obesity have higher serum alanine aminotransferase, C-reacti2021-01-01T00:00:00Z2021 Mar2026-05-30T16:51:10.885Z2024-12-04T03:36:02.046ZS-EPMC79277493362443810.1002/oby.23081