biostudies-literatureUnknown9Liu ZJiangsu Agricultural Science and Technology Independent Innovation FundNational Natural Science Foundation of ChinaGenome mining is more and more widely used in identifying new enzymes from database. In the present study, we reported a putative xylanase, Pg-Xyn (WP_053166147.1), which originated from a psychrotolerant strain <i>Planomicrobium glaciei</i> CHR 43, and was identified from Genbank by genome mining. Sequence analysis and homology modeling showed that Pg-Xyn belongs to glycosyl hydrolase family 10. On the basis of heterologous expression in <i>E. coli</i> and biochemical characterization, we found Pg-Xyn was most active at pH 9.0 and 80°C and exhibited good stability from pH 5.0 to 12.0 and below 90°C. Pg-Xyn was slightly activated in the presence of Ca²⁺ and Mg²⁺, while it was strongly inhibited by Mn²⁺. The analysis of hydrolysis products showed that Pg-XynFrontiers in bioengineering and biotechnology618979https://www.ebi.ac.uk/biostudies/studies/S-EPMC7928332biostudies-literatureExpression, Characterization and Its Deinking Potential of a Thermostable Xylanase From <i>Planomicrobium glaciei</i> CHR43.PMC7928332Hao NLiu ZLi YWu BJia HShao TfalseExpression, Characterization and Its Deinking Potential of a Thermostable Xylanase From <i>Planomicrobium glaciei</i> CHR43.Genome mining is more and more widely used in identifying new enzymes from database. In the present study, we reported a putative xylanase, Pg-Xyn (WP_053166147.1), which originated from a psychrotolerant strain <i>Planomicrobium glaciei</i> CHR 43, and was identified from Genbank by genome mining. Sequence analysis and homology modeling showed that Pg-Xyn belongs to glycosyl hydrolase family 10. On the basis of heterologous expression in <i>E. coli</i> and biochemical characterization, we found Pg-Xyn was most active at pH 9.0 and 80°C and exhibited good stability from pH 5.0 to 12.0 and below 90°C. Pg-Xyn was slightly activated in the presence of Ca²⁺ and Mg²⁺, while it was strongly inhibited by Mn²⁺. The analysis of hydrolysis products showed that Pg-Xyn2021-01-01T00:00:00Z20212026-05-09T08:57:48.864Z2021-03-12T08:16:20ZS-EPMC79283323368115810.3389/fbioe.2021.618979