{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["22"],"submitter":["Costa ADT"],"funding":["Banco Nacional de Desenvolvimento Econômico e Social"],"pubmed_abstract":["Foodborne outbreaks caused by parasites have long been a public health issue. Among the available contamination detection methods, qPCR is one of the most sensitive and specific. However, it can be cumbersome and error-prone, if used by unexperienced users. Moreover, qPCR reagents usually require freezer temperatures for transportation and storage. We present a gelified reaction format that allows the reagents to be stored at 2-8 °C for up to 90 days without losing performance. The gelification process eliminates most operator mistakes during reaction setup, and renders the qPCR plates ready-to-use. The new reaction makeup was evaluated using artificially contaminated samples of distinct food matrices for sensitivity, specificity, repeatability, reproducibility, and stability. Samples cons"],"journal":["Food and waterborne parasitology"],"pagination":["e00111"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7930119"],"repository":["biostudies-literature"],"pubmed_title":["Ready-to-use qPCR for detection of <i>Cyclospora cayetanensis</i> or <i>Trypanosoma cruzi</i> in food matrices."],"pmcid":["PMC7930119"],"pubmed_authors":["Pereira-Chioccola VL","Costa ADT","da Silva AJ","Rampazzo RCP","Pinto RS","Murphy HR","Krieger MA","Tassi W","Farias AB","Mattos EC","Jacomasso T","Marciano MAM"],"additional_accession":[]},"is_claimable":false,"name":"Ready-to-use qPCR for detection of <i>Cyclospora cayetanensis</i> or <i>Trypanosoma cruzi</i> in food matrices.","description":"Foodborne outbreaks caused by parasites have long been a public health issue. Among the available contamination detection methods, qPCR is one of the most sensitive and specific. However, it can be cumbersome and error-prone, if used by unexperienced users. Moreover, qPCR reagents usually require freezer temperatures for transportation and storage. We present a gelified reaction format that allows the reagents to be stored at 2-8 °C for up to 90 days without losing performance. The gelification process eliminates most operator mistakes during reaction setup, and renders the qPCR plates ready-to-use. The new reaction makeup was evaluated using artificially contaminated samples of distinct food matrices for sensitivity, specificity, repeatability, reproducibility, and stability. Samples cons","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2026-04-07T23:27:13.681Z","creation":"2021-03-09T08:18:11Z"},"accession":"S-EPMC7930119","cross_references":{"pubmed":["33681489"],"doi":["10.1016/j.fawpar.2021.e00111"]}}