biostudies-literatureSedlmeier EMTechnische Universität MünchenElse Kröner-Fresenius Foundation, Bad Homburg, GermanyGerman Ministry of Education and Research via the Competence Network Obesity (Kompetenznetz Adipositas), GermanyInstitut DANONE Ernährung und Gesundheit e.V., Haar, Germany15https://www.ebi.ac.uk/biostudies/studies/S-EPMC7931339biostudies-literatureUnknown22(1)<h4>Background</h4>Previously, we revealed sexually dimorphic mRNA expression and responsiveness to maternal dietary supplementation with n-3 long-chain polyunsaturated fatty acids (LCPUFA) in placentas from a defined INFAT study subpopulation. Here, we extended these analyses and explored the respective placental microRNA expression, putative microRNA-mRNA interactions, and downstream target processes as well as their associations with INFAT offspring body composition.<h4>Results</h4>We performed explorative placental microRNA profiling, predicted microRNA-mRNA interactions by bioinformatics, validated placental target microRNAs and their putative targets by RT-qPCR and western blotting, and measured amino acid levels in maternal and offspring cord blood plasma and placenta. microRNA, mRNBMC molecular and cell biologyFetal sex modulates placental microRNA expression, potential microRNA-mRNA interactions, and levels of amino acid transporter expression and substrates: INFAT study subpopulation analysis of n-3 LCPUFA intervention during pregnancy and associations with offspring body composition.PMC793133901GI0842Project# 2011/20Hauner HStecher LMeyer DMSailer MBader BLSedlmeier EMDaniel HfalseFetal sex modulates placental microRNA expression, potential microRNA-mRNA interactions, and levels of amino acid transporter expression and substrates: INFAT study subpopulation analysis of n-3 LCPUFA intervention during pregnancy and associations with offspring body composition.<h4>Background</h4>Previously, we revealed sexually dimorphic mRNA expression and responsiveness to maternal dietary supplementation with n-3 long-chain polyunsaturated fatty acids (LCPUFA) in placentas from a defined INFAT study subpopulation. Here, we extended these analyses and explored the respective placental microRNA expression, putative microRNA-mRNA interactions, and downstream target processes as well as their associations with INFAT offspring body composition.<h4>Results</h4>We performed explorative placental microRNA profiling, predicted microRNA-mRNA interactions by bioinformatics, validated placental target microRNAs and their putative targets by RT-qPCR and western blotting, and measured amino acid levels in maternal and offspring cord blood plasma and placenta. microRNA, mRN2021-01-01T00:00:00Z2021 Mar2026-05-03T08:53:23.3Z2021-03-09T08:18:53ZS-EPMC79313393365799210.1186/s12860-021-00345-x