{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(1)"],"submitter":["Bokelmann L"],"funding":["Funding was provided by the Max Planck Society, the NOMIS foundation and the ImmunoDeficiencyCenter Leipzig"],"pubmed_abstract":["Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than"],"journal":["Nature communications"],"pagination":["1467"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7935920"],"repository":["biostudies-literature"],"pubmed_title":["Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP."],"pmcid":["PMC7935920"],"pubmed_authors":["Nickel O","Riesenberg S","Maricic T","Borte S","Meyer M","Paabo S","Bokelmann L"],"additional_accession":[]},"is_claimable":false,"name":"Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP.","description":"Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2025-04-22T02:08:55.265Z","creation":"2025-04-05T20:12:18.715Z"},"accession":"S-EPMC7935920","cross_references":{"pubmed":["33674580"],"doi":["10.1038/s41467-021-21627-0"]}}