biostudies-literatureUnknown12(1)Bokelmann LFunding was provided by the Max Planck Society, the NOMIS foundation and the ImmunoDeficiencyCenter LeipzigEfforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less thanNature communications1467https://www.ebi.ac.uk/biostudies/studies/S-EPMC7935920biostudies-literaturePoint-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP.PMC7935920Nickel ORiesenberg SMaricic TBorte SMeyer MPaabo SBokelmann LfalsePoint-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP.Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than2021-01-01T00:00:00Z2021 Mar2025-04-22T02:08:55.265Z2025-04-05T20:12:18.715ZS-EPMC79359203367458010.1038/s41467-021-21627-0