{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["21(4)"],"submitter":["Li Y"],"pubmed_abstract":["Osteoarthritis (OA), characterized by the degeneration of articular cartilage, is a major problem in aging populations, and cartilage chondrocytes have been indicated to serve a curial role in the progression of OA. MicroRNA-200b-3p (miR-200b) was preliminarily identified to participate in OA. However, its role and mechanism of action in injured chondrocytes in OA remain unclear to date. In the present study, lipopolysaccharide (LPS)-treated cells isolated from normal knee articular cartilage were used to mimic inflammatory injury of OA chondrocytes. Cell viability, apoptosis and inflammatory responses were detected using Cell Counting Kit-8, flow cytometry and enzyme-linked immunosorbent assay, respectively. The expression levels of miR-200b and fucosyltransferase-4 (FUT4) were measured b"],"journal":["Experimental and therapeutic medicine"],"pagination":["407"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7938448"],"repository":["biostudies-literature"],"pubmed_title":["MicroRNA-200b relieves LPS-induced inflammatory injury by targeting FUT4 in knee articular chondrocytes <i>in vitro</i>."],"pmcid":["PMC7938448"],"pubmed_authors":["Li Y","Zhang Z","Wei S"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA-200b relieves LPS-induced inflammatory injury by targeting FUT4 in knee articular chondrocytes <i>in vitro</i>.","description":"Osteoarthritis (OA), characterized by the degeneration of articular cartilage, is a major problem in aging populations, and cartilage chondrocytes have been indicated to serve a curial role in the progression of OA. MicroRNA-200b-3p (miR-200b) was preliminarily identified to participate in OA. However, its role and mechanism of action in injured chondrocytes in OA remain unclear to date. In the present study, lipopolysaccharide (LPS)-treated cells isolated from normal knee articular cartilage were used to mimic inflammatory injury of OA chondrocytes. Cell viability, apoptosis and inflammatory responses were detected using Cell Counting Kit-8, flow cytometry and enzyme-linked immunosorbent assay, respectively. The expression levels of miR-200b and fucosyltransferase-4 (FUT4) were measured b","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2025-04-21T18:10:38.91Z","creation":"2021-03-13T08:20:26Z"},"accession":"S-EPMC7938448","cross_references":{"pubmed":["33692838"],"doi":["10.3892/etm.2021.9838"]}}