biostudies-literatureUnknown21(4)Li YOsteoarthritis (OA), characterized by the degeneration of articular cartilage, is a major problem in aging populations, and cartilage chondrocytes have been indicated to serve a curial role in the progression of OA. MicroRNA-200b-3p (miR-200b) was preliminarily identified to participate in OA. However, its role and mechanism of action in injured chondrocytes in OA remain unclear to date. In the present study, lipopolysaccharide (LPS)-treated cells isolated from normal knee articular cartilage were used to mimic inflammatory injury of OA chondrocytes. Cell viability, apoptosis and inflammatory responses were detected using Cell Counting Kit-8, flow cytometry and enzyme-linked immunosorbent assay, respectively. The expression levels of miR-200b and fucosyltransferase-4 (FUT4) were measured bExperimental and therapeutic medicine407https://www.ebi.ac.uk/biostudies/studies/S-EPMC7938448biostudies-literatureMicroRNA-200b relieves LPS-induced inflammatory injury by targeting FUT4 in knee articular chondrocytes <i>in vitro</i>.PMC7938448Li YZhang ZWei SfalseMicroRNA-200b relieves LPS-induced inflammatory injury by targeting FUT4 in knee articular chondrocytes <i>in vitro</i>.Osteoarthritis (OA), characterized by the degeneration of articular cartilage, is a major problem in aging populations, and cartilage chondrocytes have been indicated to serve a curial role in the progression of OA. MicroRNA-200b-3p (miR-200b) was preliminarily identified to participate in OA. However, its role and mechanism of action in injured chondrocytes in OA remain unclear to date. In the present study, lipopolysaccharide (LPS)-treated cells isolated from normal knee articular cartilage were used to mimic inflammatory injury of OA chondrocytes. Cell viability, apoptosis and inflammatory responses were detected using Cell Counting Kit-8, flow cytometry and enzyme-linked immunosorbent assay, respectively. The expression levels of miR-200b and fucosyltransferase-4 (FUT4) were measured b2021-01-01T00:00:00Z2021 Apr2025-04-21T18:10:38.91Z2021-03-13T08:20:26ZS-EPMC79384483369283810.3892/etm.2021.9838