{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kim YS"],"funding":["National Research Foundation of Korea"],"pagination":["e12996"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7941225"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["54(3)"],"pubmed_abstract":["<h4>Objectives</h4>The female reproductive tract comprises several different cell types. Using three representative Cre systems, we comparatively analysed the phenotypes of Dgcr8 conditional knockout (cKO) mice to understand the function of Dgcr8, involved in canonical microRNA biogenesis, in the female reproductive tract.<h4>Materials and methods</h4>Dgcr8<sup>f/f</sup> mice were crossed with Ltf<sup>icre/+</sup> , Amhr2<sup>cre/+</sup> or PR<sup>cre/+</sup> mice to produce mice deficient in Dgcr8 in epithelial (Dgcr8<sup>ed/ed</sup> ), mesenchymal (Dgcr8<sup>md/md</sup> ) and all the compartments (Dgcr8<sup>td/td</sup> ) in the female reproductive tract. Reproductive phenotypes were evaluated in Dgcr8 cKO mice. Uteri and/or oviducts were used for small RNA-seq, mRNA-seq, real-time RT-PCR"],"journal":["Cell proliferation"],"pubmed_title":["Different Cre systems induce differential microRNA landscapes and abnormalities in the female reproductive tracts of Dgcr8 conditional knockout mice."],"pmcid":["PMC7941225"],"funding_grant_id":["2020R1A2C2005012","2019R1A6A1A03032888","2020R1A2C1004122","2016R1C1B1015648"],"pubmed_authors":["Yang SC","Park M","Lim HJ","Choi Y","Song H","Kim YS","Lydon JP","Kim HR","DeMayo FJ"],"additional_accession":[]},"is_claimable":false,"name":"Different Cre systems induce differential microRNA landscapes and abnormalities in the female reproductive tracts of Dgcr8 conditional knockout mice.","description":"<h4>Objectives</h4>The female reproductive tract comprises several different cell types. Using three representative Cre systems, we comparatively analysed the phenotypes of Dgcr8 conditional knockout (cKO) mice to understand the function of Dgcr8, involved in canonical microRNA biogenesis, in the female reproductive tract.<h4>Materials and methods</h4>Dgcr8<sup>f/f</sup> mice were crossed with Ltf<sup>icre/+</sup> , Amhr2<sup>cre/+</sup> or PR<sup>cre/+</sup> mice to produce mice deficient in Dgcr8 in epithelial (Dgcr8<sup>ed/ed</sup> ), mesenchymal (Dgcr8<sup>md/md</sup> ) and all the compartments (Dgcr8<sup>td/td</sup> ) in the female reproductive tract. Reproductive phenotypes were evaluated in Dgcr8 cKO mice. Uteri and/or oviducts were used for small RNA-seq, mRNA-seq, real-time RT-PCR","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2025-04-19T17:47:26.053Z","creation":"2025-04-19T17:47:26.053Z"},"accession":"S-EPMC7941225","cross_references":{"pubmed":["33496365"],"doi":["10.1111/cpr.12996"]}}