biostudies-literatureLiang QOpen Foundation of Shandong Provincial Key Laboratory of Oral Tissue RegenerationNational Key R&D Program of ChinaNational Natural Science Foundation of ChinaCollaborative Innovation Center of Technology and Equipment for Biological Diagnosis and Therapy in Universities of Shandong, The National Key Research and Development Program of ChinaConstruction Engineering Special Fund of "Taishan Scholars" of Shandong Provincee12997https://www.ebi.ac.uk/biostudies/studies/S-EPMC7941242biostudies-literatureUnknown54(3)<h4>Objectives</h4>Stromal cell-derived factor-1 (SDF-1) actively directs endogenous cell homing. Exendin-4 (EX-4) promotes stem cell osteogenic differentiation. Studies revealed that EX-4 strengthened SDF-1-mediated stem cell migration. However, the effects of SDF-1 and EX-4 on periodontal ligament stem cells (PDLSCs) and bone regeneration have not been investigated. In this study, we aimed to evaluate the effects of SDF-1/EX-4 cotherapy on PDLSCs in vitro and periodontal bone regeneration in vivo.<h4>Methods</h4>Cell-counting kit-8 (CCK8), transwell assay, qRT-PCR and western blot were used to determine the effects and mechanism of SDF-1/EX-4 cotherapy on PDLSCs in vitro. A rat periodontal bone defect model was developed to evaluate the effects of topical application of SDF-1 and systemiCell proliferationStromal cell-derived factor-1/Exendin-4 cotherapy facilitates the proliferation, migration and osteogenic differentiation of human periodontal ligament stem cells in vitro and promotes periodontal bone regeneration in vivo.PMC7941242818737162017YFA0104604SDKQ201901tsqn201909180SDKQ201904816709932017YFB0405400ts20190975Ge SKang WDu LZhang RLiang QfalseStromal cell-derived factor-1/Exendin-4 cotherapy facilitates the proliferation, migration and osteogenic differentiation of human periodontal ligament stem cells in vitro and promotes periodontal bone regeneration in vivo.<h4>Objectives</h4>Stromal cell-derived factor-1 (SDF-1) actively directs endogenous cell homing. Exendin-4 (EX-4) promotes stem cell osteogenic differentiation. Studies revealed that EX-4 strengthened SDF-1-mediated stem cell migration. However, the effects of SDF-1 and EX-4 on periodontal ligament stem cells (PDLSCs) and bone regeneration have not been investigated. In this study, we aimed to evaluate the effects of SDF-1/EX-4 cotherapy on PDLSCs in vitro and periodontal bone regeneration in vivo.<h4>Methods</h4>Cell-counting kit-8 (CCK8), transwell assay, qRT-PCR and western blot were used to determine the effects and mechanism of SDF-1/EX-4 cotherapy on PDLSCs in vitro. A rat periodontal bone defect model was developed to evaluate the effects of topical application of SDF-1 and systemi2021-01-01T00:00:00Z2021 Mar2025-04-18T18:58:09.933Z2025-04-07T06:43:19.788ZS-EPMC79412423351170810.1111/cpr.12997