{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hsu TY"],"funding":["Ministry of Science and Technology, Taiwan","NIDCD NIH HHS","NINDS NIH HHS","National Institutes of Health","National Chiao Tung University"],"pagination":["e62449"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7946423"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10"],"pubmed_abstract":["<i>Caenorhabditis elegans</i> expresses human Werner syndrome protein (WRN) orthologs as two distinct proteins: MUT-7, with a 3'-5' exonuclease domain, and CeWRN-1, with helicase domains. How these domains cooperate remains unclear. Here, we demonstrate the different contributions of MUT-7 and CeWRN-1 to 22G small interfering RNA (siRNA) synthesis and the plasticity of neuronal signaling. MUT-7 acts specifically in the cytoplasm to promote siRNA biogenesis and in the nucleus to associate with CeWRN-1. The import of siRNA by the nuclear Argonaute NRDE-3 promotes the loading of the heterochromatin-binding protein HP1 homolog HPL-2 onto specific loci. This heterochromatin complex represses the gene expression of the guanylyl cyclase ODR-1 to direct olfactory plasticity in <i>C. elegans</i>. O"],"journal":["eLife"],"pubmed_title":["<i>C. elegans</i> orthologs MUT-7/CeWRN-1 of Werner syndrome protein regulate neuronal plasticity."],"pmcid":["PMC7946423"],"funding_grant_id":["105-2311-B-009 -002-MY3","R01 DC005991","The higher education sprout project of the National Chiao Tung University and Ministry of Education, Taiwan","103-2311-B-009 -003-MY2","R01 NS087544","2R01DC005991","R01 DC015758"],"pubmed_authors":["Juang BT","L'Etoile ND","Hsu TY","Zhang B"],"additional_accession":[]},"is_claimable":false,"name":"<i>C. elegans</i> orthologs MUT-7/CeWRN-1 of Werner syndrome protein regulate neuronal plasticity.","description":"<i>Caenorhabditis elegans</i> expresses human Werner syndrome protein (WRN) orthologs as two distinct proteins: MUT-7, with a 3'-5' exonuclease domain, and CeWRN-1, with helicase domains. How these domains cooperate remains unclear. Here, we demonstrate the different contributions of MUT-7 and CeWRN-1 to 22G small interfering RNA (siRNA) synthesis and the plasticity of neuronal signaling. MUT-7 acts specifically in the cytoplasm to promote siRNA biogenesis and in the nucleus to associate with CeWRN-1. The import of siRNA by the nuclear Argonaute NRDE-3 promotes the loading of the heterochromatin-binding protein HP1 homolog HPL-2 onto specific loci. This heterochromatin complex represses the gene expression of the guanylyl cyclase ODR-1 to direct olfactory plasticity in <i>C. elegans</i>. O","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2026-05-08T06:10:37.525Z","creation":"2021-03-16T08:12:40Z"},"accession":"S-EPMC7946423","cross_references":{"pubmed":["33646120"],"doi":["10.7554/eLife.62449"]}}