biostudies-literatureZhang MTai’shan Scholar Engineering Construction Fund of Shandong Province of ChinaShandong University647611https://www.ebi.ac.uk/biostudies/studies/S-EPMC7947916biostudies-literatureUnknown12<h4>Background</h4>Dental caries is an acid-related disease. Current anti-caries agents mainly focus on the bacteriostatic effect in a neutral environment and do not target acid-resistant microorganisms related to caries in acidic milieus.<h4>Objectives</h4>To assess the <i>in vitro</i> antibacterial activities of bedaquiline against oral pathogens in acidic milieus.<h4>Methods</h4><i>Streptococcus mutans</i>, <i>Streptococcus sanguinis</i>, and <i>Streptococcus salivarius</i> were used to prepare the mono-/multiple suspension and biofilm. The MIC and IC₅₀ of bedaquiline against <i>S. mutans</i> were determined by the broth microdilution method. Bedaquiline was compared regarding (i) the inhibitory activity in pH 4-7 and at different time points against planktonic and biofilm; (Frontiers in microbiology<i>In vitro</i> Antibacterial Activity of an FDA-Approved H⁺-ATPase Inhibitor, Bedaquiline, Against <i>Streptococcus mutans</i> in Acidic Milieus.PMC79479162018GN024TS201511106Zhang DZhou SYu WZhang MXu XZhang BLo ECMfalse<i>In vitro</i> Antibacterial Activity of an FDA-Approved H⁺-ATPase Inhibitor, Bedaquiline, Against <i>Streptococcus mutans</i> in Acidic Milieus.<h4>Background</h4>Dental caries is an acid-related disease. Current anti-caries agents mainly focus on the bacteriostatic effect in a neutral environment and do not target acid-resistant microorganisms related to caries in acidic milieus.<h4>Objectives</h4>To assess the <i>in vitro</i> antibacterial activities of bedaquiline against oral pathogens in acidic milieus.<h4>Methods</h4><i>Streptococcus mutans</i>, <i>Streptococcus sanguinis</i>, and <i>Streptococcus salivarius</i> were used to prepare the mono-/multiple suspension and biofilm. The MIC and IC₅₀ of bedaquiline against <i>S. mutans</i> were determined by the broth microdilution method. Bedaquiline was compared regarding (i) the inhibitory activity in pH 4-7 and at different time points against planktonic and biofilm; (2021-01-01T00:00:00Z20212026-05-02T16:47:21.782Z2021-03-14T08:17:18ZS-EPMC79479163371704610.3389/fmicb.2021.647611