biostudies-literatureUnknown20Martinez-Huenchullan SFCancer Institute NSWNational Health and Medical Research CouncilThe increasing consumption of high-fat foods combined with a lack of exercise is a major contributor to the burden of obesity in humans. Aerobic exercise such as running is known to provide metabolic benefits, but how the overconsumption of a high-fat diet (HFD) and exercise interact is not well characterized at the molecular level. Here, we examined the plasma proteome in mice for the effects of aerobic exercise as both a treatment and as a preventative regimen for animals on either a HFD or a healthy control diet. This analysis detected large changes in the plasma proteome induced by the HFD, such as increased abundance of SERPINA7, ALDOB, and downregulation of SERPINA1E and complement factor D (CFD; adipsin). Some of these changes were significantly reverted using exercise as a preventaMolecular & cellular proteomics : MCP100027https://www.ebi.ac.uk/biostudies/studies/S-EPMC7950115biostudies-literatureBlockade of High-Fat Diet Proteomic Phenotypes Using Exercise as Prevention or Treatment.PMC7950115Larance MMin DTwigg SMMartinez-Huenchullan SFHatchwell LShipsey IfalseBlockade of High-Fat Diet Proteomic Phenotypes Using Exercise as Prevention or Treatment.The increasing consumption of high-fat foods combined with a lack of exercise is a major contributor to the burden of obesity in humans. Aerobic exercise such as running is known to provide metabolic benefits, but how the overconsumption of a high-fat diet (HFD) and exercise interact is not well characterized at the molecular level. Here, we examined the plasma proteome in mice for the effects of aerobic exercise as both a treatment and as a preventative regimen for animals on either a HFD or a healthy control diet. This analysis detected large changes in the plasma proteome induced by the HFD, such as increased abundance of SERPINA7, ALDOB, and downregulation of SERPINA1E and complement factor D (CFD; adipsin). Some of these changes were significantly reverted using exercise as a preventa2021-01-01T00:00:00Z20212026-05-09T08:40:08.307Z2022-02-10T09:10:15.514ZS-EPMC79501153359498910.1074/mcp.TIR120.002343