{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang X"],"funding":["Shanghai Municipal People's Government","National Natural Science Foundation of China","National Key Research and Development Program of China"],"pagination":["100023"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7950199"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20"],"pubmed_abstract":["Gonadal soma-derived factor (gsdf) has been demonstrated to be essential for testicular differentiation in medaka (Oryzias latipes). To understand the protein dynamics of Gsdf in spermatogenesis regulation, we used a His-tag \"pull-down\" assay coupled with shotgun LC-MS/MS to identify a group of potential interacting partners for Gsdf, which included cytoplasmic dynein light chain 2, eukaryotic polypeptide elongation factor 1 alpha (eEF1α), and actin filaments in the mature medaka testis. As for the interaction with transforming growth factor β-dynein being critical for spermatogonial division in Drosophila melanogaster, the physical interactions of Gsdf-dynein and Gsdf-eEF1α were identified through a yeast 2-hybrid screening of an adult testis cDNA library using Gsdf as bait, which were ve"],"journal":["Molecular & cellular proteomics : MCP"],"pubmed_title":["A Potential Role for the Gsdf-eEF1α Complex in Inhibiting Germ Cell Proliferation: A Protein-Interaction Analysis in Medaka (Oryzias latipes) From a Proteomics Perspective."],"pmcid":["PMC7950199"],"funding_grant_id":["81771545","2018YFD0900601"],"pubmed_authors":["Guan G","Qian F","Chen L","Zhong X","Chang Y","Zhao X","Zhai W","Zhang Y","Guo H","Wang S","Zhang X","Xu S","Hu R"],"additional_accession":[]},"is_claimable":false,"name":"A Potential Role for the Gsdf-eEF1α Complex in Inhibiting Germ Cell Proliferation: A Protein-Interaction Analysis in Medaka (Oryzias latipes) From a Proteomics Perspective.","description":"Gonadal soma-derived factor (gsdf) has been demonstrated to be essential for testicular differentiation in medaka (Oryzias latipes). To understand the protein dynamics of Gsdf in spermatogenesis regulation, we used a His-tag \"pull-down\" assay coupled with shotgun LC-MS/MS to identify a group of potential interacting partners for Gsdf, which included cytoplasmic dynein light chain 2, eukaryotic polypeptide elongation factor 1 alpha (eEF1α), and actin filaments in the mature medaka testis. As for the interaction with transforming growth factor β-dynein being critical for spermatogonial division in Drosophila melanogaster, the physical interactions of Gsdf-dynein and Gsdf-eEF1α were identified through a yeast 2-hybrid screening of an adult testis cDNA library using Gsdf as bait, which were ve","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2026-05-07T22:17:04.901Z","creation":"2024-11-13T06:30:46.674Z"},"accession":"S-EPMC7950199","cross_references":{"pubmed":["33293461"],"doi":["10.1074/mcp.RA120.002306"]}}