{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Foscari A"],"funding":["Università degli Studi di Udine"],"pagination":["e0247842"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7951814"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(3)"],"pubmed_abstract":["In last decades, a large body of evidence clarified nitrogen isotope composition (δ15N) patterns in plant leaves, roots and metabolites, showing isotopic fractionation along N uptake and assimilation pathways, in relation to N source and use efficiency, also suggesting 15N depletion in plant DNA. Here we present a manipulative experiment on Brassica napus var. oleracea, where we monitored δ 15N of purified, lyophilized DNA and source leaf and root materials, over a 60-days growth period starting at d 60 after germination, in plants initially supplied with a heavy labelled (δ 15NAir-N2 = 2100 mUr) ammonium nitrate solution covering nutrient requirements for the whole observation period (470 mg N per plant) and controlling for the labelled N species (ṄH4, ṄO3 and both). Dynamics of Isotopic "],"journal":["PloS one"],"pubmed_title":["N uptake, assimilation and isotopic fractioning control δ 15N dynamics in plant DNA: A heavy labelling experiment on Brassica napus L."],"pmcid":["PMC7951814"],"funding_grant_id":["PRID2017"],"pubmed_authors":["Foscari A","Incerti G","Leonarduzzi G"],"additional_accession":[]},"is_claimable":false,"name":"N uptake, assimilation and isotopic fractioning control δ 15N dynamics in plant DNA: A heavy labelling experiment on Brassica napus L.","description":"In last decades, a large body of evidence clarified nitrogen isotope composition (δ15N) patterns in plant leaves, roots and metabolites, showing isotopic fractionation along N uptake and assimilation pathways, in relation to N source and use efficiency, also suggesting 15N depletion in plant DNA. Here we present a manipulative experiment on Brassica napus var. oleracea, where we monitored δ 15N of purified, lyophilized DNA and source leaf and root materials, over a 60-days growth period starting at d 60 after germination, in plants initially supplied with a heavy labelled (δ 15NAir-N2 = 2100 mUr) ammonium nitrate solution covering nutrient requirements for the whole observation period (470 mg N per plant) and controlling for the labelled N species (ṄH4, ṄO3 and both). Dynamics of Isotopic ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2025-06-01T02:32:09.5Z","creation":"2025-06-01T02:32:09.5Z"},"accession":"S-EPMC7951814","cross_references":{"pubmed":["33705458"],"doi":["10.1371/journal.pone.0247842"]}}