{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Seiron P"],"funding":["Juvenile Diabetes Foundation International","Novo Nordisk Foundation","The Ernfors Family Fund","Vetenskapsrådet","EFSD/Novo Nordisk Grant","Diabetes Wellness Foundation Sweden Junior Grants","Tore Nilsson foundation","Magnus Bergvall foundation","Sten A Olsson foundation","Barndiabetesfonden","Diabetesfonden","Åke Wiberg Stiftelse","Åke Wiberg foundation","Tore Nilsons Stiftelse för Medicinsk Forskning","Helmsley Charitable Trust","Novo Nordisk Fonden","Swedish Diabetes Association"],"pagination":["e0247888"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7954335"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(3)"],"pubmed_abstract":["Insulin secretion is impaired with increasing age. In this study, we aimed to determine whether aging induces specific transcriptional changes in human islets. Laser capture microdissection was used to extract pancreatic islet tissue from 37 deceased organ donors aged 1-81 years. The transcriptomes of the extracted islets were analysed using Ion AmpliSeq sequencing. 346 genes that co-vary significantly with age were found. There was an increased transcription of genes linked to senescence, and several aspects of the cell cycle machinery were downregulated with increasing age. We detected numerous genes not linked to aging in previous studies likely because earlier studies analysed islet cells isolated by enzymatic digestion which might affect the islet transcriptome. Among the novel genes "],"journal":["PloS one"],"pubmed_title":["Transcriptional analysis of islets of Langerhans from organ donors of different ages."],"pmcid":["PMC7954335"],"funding_grant_id":["NNF18OC0034388","NNF14OC0010935","NNF20OC0063600","NNF16OC0021334","K2015-54X-12219-19-4","NNF17OC0027314","720-747 JDWG","65X-12219-15-6","2019-01415"],"pubmed_authors":["Lundberg M","Hedin A","Volkov P","Seiron P","Esguerra JLS","Korsgren O","Stenwall A","Granlund L","Skog O","Renstrom E"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptional analysis of islets of Langerhans from organ donors of different ages.","description":"Insulin secretion is impaired with increasing age. In this study, we aimed to determine whether aging induces specific transcriptional changes in human islets. Laser capture microdissection was used to extract pancreatic islet tissue from 37 deceased organ donors aged 1-81 years. The transcriptomes of the extracted islets were analysed using Ion AmpliSeq sequencing. 346 genes that co-vary significantly with age were found. There was an increased transcription of genes linked to senescence, and several aspects of the cell cycle machinery were downregulated with increasing age. We detected numerous genes not linked to aging in previous studies likely because earlier studies analysed islet cells isolated by enzymatic digestion which might affect the islet transcriptome. Among the novel genes ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2026-04-13T03:12:36.906Z","creation":"2022-02-09T17:56:58.064Z"},"accession":"S-EPMC7954335","cross_references":{"pubmed":["33711030"],"doi":["10.1371/journal.pone.0247888"]}}