biostudies-literatureUhlorn JANIDDK NIH HHSNHLBI NIH HHSe018038https://www.ebi.ac.uk/biostudies/studies/S-EPMC7955317biostudies-literatureUnknown10(2)Background Menopause is associated with an increase in the prevalence and severity of hypertension in women. Although premenopausal females are protected against T cell-dependent immune activation and development of angiotensin II (Ang II) hypertension, this protection is lost in postmenopausal females. Therefore, the current study hypothesized that specific CD4⁺ T cell pathways are regulated by sex hormones and Ang II to mediate progression from premenopausal protection to postmenopausal hypertension. Methods and Results Menopause was induced in C57BL/6 mice via repeated 4-vinylcyclohexene diepoxide injections, while premenopausal females received sesame oil vehicle. A subset of premenopausal mice and all menopausal mice were infused with Ang II for 14 days (Control, Ang II, MeJournal of the American Heart AssociationCD4⁺ T Cell-Specific Proteomic Pathways Identified in Progression of Hypertension Across Postmenopausal Transition.PMC7955317T32 HL007249R01 HL131834R01 DK098493Moffett CHusband NAUhlorn JALindsey MLLanglais PRRomero-Aleshire MJBrooks HLfalseCD4⁺ T Cell-Specific Proteomic Pathways Identified in Progression of Hypertension Across Postmenopausal Transition.Background Menopause is associated with an increase in the prevalence and severity of hypertension in women. Although premenopausal females are protected against T cell-dependent immune activation and development of angiotensin II (Ang II) hypertension, this protection is lost in postmenopausal females. Therefore, the current study hypothesized that specific CD4⁺ T cell pathways are regulated by sex hormones and Ang II to mediate progression from premenopausal protection to postmenopausal hypertension. Methods and Results Menopause was induced in C57BL/6 mice via repeated 4-vinylcyclohexene diepoxide injections, while premenopausal females received sesame oil vehicle. A subset of premenopausal mice and all menopausal mice were infused with Ang II for 14 days (Control, Ang II, Me2021-01-01T00:00:00Z2021 Jan2026-04-18T01:04:23.148Z2024-12-03T16:37:59.056ZS-EPMC79553173341033310.1161/JAHA.120.018038