biostudies-literatureUnknown9(24)Frati GBackground The role of microRNAs dysregulation in tobacco cigarette smoking-induced vascular damage still needs to be clarified. We assessed the acute effects of tobacco cigarette smoking on endothelial cell-related circulating microRNAs in healthy subjects. In addition, we investigated the potential role of microRNAs in smoking-dependent endothelial cell damage. Methods and Results A panel of endothelial-related microRNAs was quantified in healthy subjects before and after smoking 1 tobacco cigarette. Serum levels of miR-155 were found to be significantly increased shortly after smoking. We also observed a progressive and significant miR-155 accumulation in culture media of human endothelial cells after 30 minutes and up to 4 hours of cigarette smoke condensate treatment in vitro without Journal of the American Heart Associatione017000https://www.ebi.ac.uk/biostudies/studies/S-EPMC7955400biostudies-literatureInhibition of miR-155 Attenuates Detrimental Vascular Effects of Tobacco Cigarette Smoking.PMC7955400di Nonno FVersaci FDe Falco ENocella CSciarretta SBianchi FCarnevale RCavarretta EPicchio VStanzione RMarchitti SSchiavon SRubattu SVecchio DChimenti IFrati GBordin APagano FPaneni FForte MVolpe MBiondi-Zoccai GfalseInhibition of miR-155 Attenuates Detrimental Vascular Effects of Tobacco Cigarette Smoking.Background The role of microRNAs dysregulation in tobacco cigarette smoking-induced vascular damage still needs to be clarified. We assessed the acute effects of tobacco cigarette smoking on endothelial cell-related circulating microRNAs in healthy subjects. In addition, we investigated the potential role of microRNAs in smoking-dependent endothelial cell damage. Methods and Results A panel of endothelial-related microRNAs was quantified in healthy subjects before and after smoking 1 tobacco cigarette. Serum levels of miR-155 were found to be significantly increased shortly after smoking. We also observed a progressive and significant miR-155 accumulation in culture media of human endothelial cells after 30 minutes and up to 4 hours of cigarette smoke condensate treatment in vitro without 2020-01-01T00:00:00Z2020 Dec2025-04-21T17:03:17.6Z2025-04-21T17:03:17.6ZS-EPMC79554003331736910.1161/JAHA.120.017000