biostudies-literatureUnknown9(1)Neo PSBrain-computer interfaces (BCIs) target specific brain activity for neuropsychological rehabilitation, and also allow patients with motor disabilities to control mobility and communication devices. Motor imagery of single-handed actions is used in BCIs but many users cannot control the BCIs effectively, limiting applications in the health systems. Crosstalk is unintended brain activations that interfere with bimanual actions and could also occur during motor imagery. To test if crosstalk impaired BCI user performance, we recorded EEG in 46 participants while they imagined movements in four experimental conditions using motor imagery: left hand (L), right hand (R), tongue (T) and feet (F). Pairwise classification accuracies of the tasks were compared (LR, LF, LT, RF, RT, FT), using common sHealth information science and systems13https://www.ebi.ac.uk/biostudies/studies/S-EPMC7956071biostudies-literatureCrosstalk disrupts the production of motor imagery brain signals in brain-computer interfaces.PMC7956071Mayne THuang ZFu XNeo PSFranz EAfalseCrosstalk disrupts the production of motor imagery brain signals in brain-computer interfaces.Brain-computer interfaces (BCIs) target specific brain activity for neuropsychological rehabilitation, and also allow patients with motor disabilities to control mobility and communication devices. Motor imagery of single-handed actions is used in BCIs but many users cannot control the BCIs effectively, limiting applications in the health systems. Crosstalk is unintended brain activations that interfere with bimanual actions and could also occur during motor imagery. To test if crosstalk impaired BCI user performance, we recorded EEG in 46 participants while they imagined movements in four experimental conditions using motor imagery: left hand (L), right hand (R), tongue (T) and feet (F). Pairwise classification accuracies of the tasks were compared (LR, LF, LT, RF, RT, FT), using common s2021-01-01T00:00:00Z2021 Dec2025-04-26T09:47:18.98Z2025-04-06T13:09:52.497ZS-EPMC79560713378616210.1007/s13755-021-00142-y