{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Engevik MA"],"funding":["National Institute of Allergy and Infectious Diseases","NIDDK NIH HHS","National Institute of Diabetes and Digestive and Kidney Diseases","NIAID NIH HHS"],"pagination":["1301-1314.e8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7956072"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["160(4)"],"pubmed_abstract":["<h4>Background & aims</h4>Although Clostridioides difficile infection (CDI) is known to involve the disruption of the gut microbiota, little is understood regarding how mucus-associated microbes interact with C difficile. We hypothesized that select mucus-associated bacteria would promote C difficile colonization and biofilm formation.<h4>Methods</h4>To create a model of the human intestinal mucus layer and gut microbiota, we used bioreactors inoculated with healthy human feces, treated with clindamycin and infected with C difficile with the addition of human MUC2-coated coverslips.<h4>Results</h4>C difficile was found to colonize and form biofilms on MUC2-coated coverslips, and 16S rRNA sequencing showed a unique biofilm profile with substantial cocolonization with Fusobacterium species. "],"journal":["Gastroenterology"],"pubmed_title":["Fusobacteriumnucleatum Adheres to Clostridioides difficile via the RadD Adhesin to Enhance Biofilm Formation in Intestinal Mucus."],"pmcid":["PMC7956072"],"funding_grant_id":["K01 DK123195","F32 AI136404","P30 DK056338","R01 AI123278","U01 AI124290","P30 DK123704"],"pubmed_authors":["Britton RA","Versalovic J","Auchtung J","Endres BT","Nicholson M","Lux R","Wu Q","Yacyshyn B","Engevik AC","Luna RA","Savidge T","Ruan W","Crawford SE","Engevik MA","Yacyshyn MB","Estes MK","Danhof HA","Basseres E","Garey KW"],"additional_accession":[]},"is_claimable":false,"name":"Fusobacteriumnucleatum Adheres to Clostridioides difficile via the RadD Adhesin to Enhance Biofilm Formation in Intestinal Mucus.","description":"<h4>Background & aims</h4>Although Clostridioides difficile infection (CDI) is known to involve the disruption of the gut microbiota, little is understood regarding how mucus-associated microbes interact with C difficile. We hypothesized that select mucus-associated bacteria would promote C difficile colonization and biofilm formation.<h4>Methods</h4>To create a model of the human intestinal mucus layer and gut microbiota, we used bioreactors inoculated with healthy human feces, treated with clindamycin and infected with C difficile with the addition of human MUC2-coated coverslips.<h4>Results</h4>C difficile was found to colonize and form biofilms on MUC2-coated coverslips, and 16S rRNA sequencing showed a unique biofilm profile with substantial cocolonization with Fusobacterium species. ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2025-04-22T01:46:18.849Z","creation":"2025-04-05T20:06:02.646Z"},"accession":"S-EPMC7956072","cross_references":{"pubmed":["33227279"],"doi":["10.1053/j.gastro.2020.11.034"]}}