{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["50(2)"],"submitter":["Jafari H"],"pubmed_abstract":["<h4>Background</h4>Most jellyfish species are poisonous. Human victims of jellyfish sting each year are 120 million. <i>Chironex fleckeri</i> is a venomous box jellyfish that inflicts painful and potentially fatal stings to humans. The CfTX-1 is one of the antigenic proteins of venom that is suggested to stimulate the immune system for treatment and vaccine. This study aimed to clone and express the CfTX-1 antigen in <i>E. coli</i> and then to determine the synthesis of related antibody in the mice.<h4>Methods</h4>The study was performed in the Persian Gulf and Oman Sea Ecology Research Center, Bandar Abbas, Iran in autumn 2016. The synthetic CfTX-1 gene in PUC57 plasmid was purchased from Nedaye Fan Company. The 723 bp fragment of N-CfTX-1 was amplified by PCR, PUC57 plasmid containing Cf"],"journal":["Iranian journal of public health"],"pagination":["376-383"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7956099"],"repository":["biostudies-literature"],"pubmed_title":["Cloning and Expression of N-CFTX-1 Antigen from <i>Chironex fleckeri</i> in <i>Escherichia coli</i> and Determination of Immunogenicity in Mice."],"pmcid":["PMC7956099"],"pubmed_authors":["Honari H","Tamadoni Jahromi S","Zargan J","Ranjbar R","Zamani E","Jafari H"],"additional_accession":[]},"is_claimable":false,"name":"Cloning and Expression of N-CFTX-1 Antigen from <i>Chironex fleckeri</i> in <i>Escherichia coli</i> and Determination of Immunogenicity in Mice.","description":"<h4>Background</h4>Most jellyfish species are poisonous. Human victims of jellyfish sting each year are 120 million. <i>Chironex fleckeri</i> is a venomous box jellyfish that inflicts painful and potentially fatal stings to humans. The CfTX-1 is one of the antigenic proteins of venom that is suggested to stimulate the immune system for treatment and vaccine. This study aimed to clone and express the CfTX-1 antigen in <i>E. coli</i> and then to determine the synthesis of related antibody in the mice.<h4>Methods</h4>The study was performed in the Persian Gulf and Oman Sea Ecology Research Center, Bandar Abbas, Iran in autumn 2016. The synthetic CfTX-1 gene in PUC57 plasmid was purchased from Nedaye Fan Company. The 723 bp fragment of N-CfTX-1 was amplified by PCR, PUC57 plasmid containing Cf","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2025-04-25T19:27:14.915Z","creation":"2025-04-06T07:58:03.576Z"},"accession":"S-EPMC7956099","cross_references":{"pubmed":["33748002"],"doi":["10.18502/ijph.v50i2.5355"]}}