biostudies-literatureUnknown50(2)Jafari H<h4>Background</h4>Most jellyfish species are poisonous. Human victims of jellyfish sting each year are 120 million. <i>Chironex fleckeri</i> is a venomous box jellyfish that inflicts painful and potentially fatal stings to humans. The CfTX-1 is one of the antigenic proteins of venom that is suggested to stimulate the immune system for treatment and vaccine. This study aimed to clone and express the CfTX-1 antigen in <i>E. coli</i> and then to determine the synthesis of related antibody in the mice.<h4>Methods</h4>The study was performed in the Persian Gulf and Oman Sea Ecology Research Center, Bandar Abbas, Iran in autumn 2016. The synthetic CfTX-1 gene in PUC57 plasmid was purchased from Nedaye Fan Company. The 723 bp fragment of N-CfTX-1 was amplified by PCR, PUC57 plasmid containing CfIranian journal of public health376-383https://www.ebi.ac.uk/biostudies/studies/S-EPMC7956099biostudies-literatureCloning and Expression of N-CFTX-1 Antigen from <i>Chironex fleckeri</i> in <i>Escherichia coli</i> and Determination of Immunogenicity in Mice.PMC7956099Honari HTamadoni Jahromi SZargan JRanjbar RZamani EJafari HfalseCloning and Expression of N-CFTX-1 Antigen from <i>Chironex fleckeri</i> in <i>Escherichia coli</i> and Determination of Immunogenicity in Mice.<h4>Background</h4>Most jellyfish species are poisonous. Human victims of jellyfish sting each year are 120 million. <i>Chironex fleckeri</i> is a venomous box jellyfish that inflicts painful and potentially fatal stings to humans. The CfTX-1 is one of the antigenic proteins of venom that is suggested to stimulate the immune system for treatment and vaccine. This study aimed to clone and express the CfTX-1 antigen in <i>E. coli</i> and then to determine the synthesis of related antibody in the mice.<h4>Methods</h4>The study was performed in the Persian Gulf and Oman Sea Ecology Research Center, Bandar Abbas, Iran in autumn 2016. The synthetic CfTX-1 gene in PUC57 plasmid was purchased from Nedaye Fan Company. The 723 bp fragment of N-CfTX-1 was amplified by PCR, PUC57 plasmid containing Cf2021-01-01T00:00:00Z2021 Feb2025-04-25T19:27:14.915Z2025-04-06T07:58:03.576ZS-EPMC79560993374800210.18502/ijph.v50i2.5355