biostudies-literatureMiao JGuangzhou Science and Technology Planning ProjectNational Natural Science Foundation of ChinaNatural Science Foundation of Guangdong Province2885-2899https://www.ebi.ac.uk/biostudies/studies/S-EPMC7957162biostudies-literatureUnknown25(6)Inflammation is a biological process that exists in a large number of diseases. If the magnitude or duration of inflammation becomes uncontrolled, inflammation may cause pathological damage to the host. HMGB1 and NF-κB have been shown to play pivotal roles in inflammation-related diseases. New drugs aimed at inhibiting HMGB1 expression have become a key research focus. In the present study, we showed that paeonol (Pae), the main active component of Paeonia suffruticosa, decreases the expression of inflammatory cytokines and inhibits the translocation of HMGB1 induced by lipopolysaccharide (LPS). By constructing HMGB1-overexpressing (HMGB1⁺ ) and HMGB1-mutant (HMGB1^m ) RAW264.7 cells, we found that the nuclear HMGB1 could induce an LPS-tolerant state in RAW264.7 cells Journal of cellular and molecular medicinePaeonol attenuates inflammation by confining HMGB1 to the nucleus.PMC7957162818731622018040 101132018B030 311014Liu XLi HLi SYe PYe SYou AChen XZhong JMiao JLan JfalsePaeonol attenuates inflammation by confining HMGB1 to the nucleus.Inflammation is a biological process that exists in a large number of diseases. If the magnitude or duration of inflammation becomes uncontrolled, inflammation may cause pathological damage to the host. HMGB1 and NF-κB have been shown to play pivotal roles in inflammation-related diseases. New drugs aimed at inhibiting HMGB1 expression have become a key research focus. In the present study, we showed that paeonol (Pae), the main active component of Paeonia suffruticosa, decreases the expression of inflammatory cytokines and inhibits the translocation of HMGB1 induced by lipopolysaccharide (LPS). By constructing HMGB1-overexpressing (HMGB1⁺ ) and HMGB1-mutant (HMGB1^m ) RAW264.7 cells, we found that the nuclear HMGB1 could induce an LPS-tolerant state in RAW264.7 cells 2021-01-01T00:00:00Z2021 Mar2025-04-22T10:45:09.523Z2025-04-05T23:42:14.675ZS-EPMC79571623353496310.1111/jcmm.16319