{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Horii M"],"funding":["NICHD NIH HHS","CIRM Research and Training grant","Bridges to Stem Cell Research Internship Program Grant from the CIRM","California Institute for Regenerative Medicine","National Institutes of Health","NIH HHS","National Institute of Child Health and Human Development","NIGMS NIH HHS","School of Medicine, CHA University, South Korea"],"pagination":["5877"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7961010"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(1)"],"pubmed_abstract":["Preeclampsia (PE) is a pregnancy-specific hypertensive disorder, affecting up to 10% of pregnancies worldwide. The primary etiology is considered to be abnormal development and function of placental cells called trophoblasts. We previously developed a two-step protocol for differentiation of human pluripotent stem cells, first into cytotrophoblast (CTB) progenitor-like cells, and then into both syncytiotrophoblast (STB)- and extravillous trophoblast (EVT)-like cells, and showed that it can model both normal and abnormal trophoblast differentiation. We have now applied this protocol to induced pluripotent stem cells (iPSC) derived from placentas of pregnancies with or without PE. While there were no differences in CTB induction or EVT formation, PE-iPSC-derived trophoblast showed a defect i"],"journal":["Scientific reports"],"pubmed_title":["Modeling preeclampsia using human induced pluripotent stem cells."],"pmcid":["PMC7961010"],"funding_grant_id":["TG2-01154 to the University of California San Diego","T32 GM008806","K99 HD091452","S10 OD026929","R01 HD089537","T32GM8806","R00 HD091452","EDUC2-08376","R01HD- 089537","RN3- 06396"],"pubmed_authors":["Rishik H","Bui T","Touma O","Parast MM","Cho HY","Laurent LC","Morey R","Horii M","Nelson KK"],"additional_accession":[]},"is_claimable":false,"name":"Modeling preeclampsia using human induced pluripotent stem cells.","description":"Preeclampsia (PE) is a pregnancy-specific hypertensive disorder, affecting up to 10% of pregnancies worldwide. The primary etiology is considered to be abnormal development and function of placental cells called trophoblasts. We previously developed a two-step protocol for differentiation of human pluripotent stem cells, first into cytotrophoblast (CTB) progenitor-like cells, and then into both syncytiotrophoblast (STB)- and extravillous trophoblast (EVT)-like cells, and showed that it can model both normal and abnormal trophoblast differentiation. We have now applied this protocol to induced pluripotent stem cells (iPSC) derived from placentas of pregnancies with or without PE. While there were no differences in CTB induction or EVT formation, PE-iPSC-derived trophoblast showed a defect i","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2025-04-20T02:29:04.594Z","creation":"2025-04-20T02:29:04.594Z"},"accession":"S-EPMC7961010","cross_references":{"pubmed":["33723311"],"doi":["10.1038/s41598-021-85230-5"]}}