{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kozar I"],"funding":["Fond National de la Recherche (FNR)","NCI NIH HHS","Fond National de la Recherche"],"pagination":["1096"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7961530"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(5)"],"pubmed_abstract":["MicroRNAs are key post-transcriptional gene regulators often displaying aberrant expression patterns in cancer. As microRNAs are promising disease-associated biomarkers and modulators of responsiveness to anti-cancer therapies, a solid understanding of their targetome is crucial. Despite enormous research efforts, the success rates of available tools to reliably predict microRNAs (miRNA)-target interactions remains limited. To investigate the disease-associated miRNA targetome, we have applied modified cross-linking ligation and sequencing of hybrids (qCLASH) to BRAF-mutant melanoma cells. The resulting RNA-RNA hybrid molecules provide a comprehensive and unbiased snapshot of direct miRNA-target interactions. The regulatory effects on selected miRNA target genes in predicted vs. non-predic"],"journal":["Cancers"],"pubmed_title":["Cross-Linking Ligation and Sequencing of Hybrids (qCLASH) Reveals an Unpredicted miRNA Targetome in Melanoma Cells."],"pmcid":["PMC7961530"],"funding_grant_id":["P01 CA214091","PRIDE15/10675146/CANBIO"],"pubmed_authors":["Gay LA","Kozar I","Philippidou D","Kreis S","Margue C","Renne R"],"additional_accession":[]},"is_claimable":false,"name":"Cross-Linking Ligation and Sequencing of Hybrids (qCLASH) Reveals an Unpredicted miRNA Targetome in Melanoma Cells.","description":"MicroRNAs are key post-transcriptional gene regulators often displaying aberrant expression patterns in cancer. As microRNAs are promising disease-associated biomarkers and modulators of responsiveness to anti-cancer therapies, a solid understanding of their targetome is crucial. Despite enormous research efforts, the success rates of available tools to reliably predict microRNAs (miRNA)-target interactions remains limited. To investigate the disease-associated miRNA targetome, we have applied modified cross-linking ligation and sequencing of hybrids (qCLASH) to BRAF-mutant melanoma cells. The resulting RNA-RNA hybrid molecules provide a comprehensive and unbiased snapshot of direct miRNA-target interactions. The regulatory effects on selected miRNA target genes in predicted vs. non-predic","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2025-04-22T10:48:39.711Z","creation":"2022-02-09T12:41:21.718Z"},"accession":"S-EPMC7961530","cross_references":{"pubmed":["33806450"],"doi":["10.3390/cancers13051096"]}}