biostudies-literatureKao YCNHLBI NIH HHS131-9https://www.ebi.ac.uk/biostudies/studies/S-EPMC7963122biostudies-literatureUnknown71Cortical spreading depolarization (CSD) is known to exacerbate ischemic damage, as the number of CSDs correlates with the final infarct volumes and suppressing CSDs improves functional outcomes. To investigate the role of CSD in ischemic damage, we developed a novel rat model of photothrombotic ischemia using a miniature implantable optic fiber that allows lesion induction inside the magnetic resonance imaging (MRI) scanner. We were able to precisely control the location and the size of the ischemic lesion, and continuously monitor dynamic perfusion and diffusion MRI signal changes at high temporal resolution before, during and after the onset of focal ischemia. Our model showed that apparent diffusion coefficient (ADC) and cerebral blood flow (CBF) in the ischemic core dropped immediatelyNeurobiology of diseaseDynamic perfusion and diffusion MRI of cortical spreading depolarization in photothrombotic ischemia.PMC7963122T32 HL069768Li WKao YCOyarzabal EALin WShih YYLai HYfalseDynamic perfusion and diffusion MRI of cortical spreading depolarization in photothrombotic ischemia.Cortical spreading depolarization (CSD) is known to exacerbate ischemic damage, as the number of CSDs correlates with the final infarct volumes and suppressing CSDs improves functional outcomes. To investigate the role of CSD in ischemic damage, we developed a novel rat model of photothrombotic ischemia using a miniature implantable optic fiber that allows lesion induction inside the magnetic resonance imaging (MRI) scanner. We were able to precisely control the location and the size of the ischemic lesion, and continuously monitor dynamic perfusion and diffusion MRI signal changes at high temporal resolution before, during and after the onset of focal ischemia. Our model showed that apparent diffusion coefficient (ADC) and cerebral blood flow (CBF) in the ischemic core dropped immediately2014-01-01T00:00:00Z2014 Nov2025-04-05T16:19:26.182Z2025-04-05T16:19:26.182ZS-EPMC79631222506677610.1016/j.nbd.2014.07.005