{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang Y"],"funding":["National Institute of Environmental Health Sciences","NIEHS NIH HHS"],"pagination":["695-698"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7965357"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["34(3)"],"pubmed_abstract":["Alkylation represents a main form of DNA damage. The <i>N</i><sup>2</sup> position of guanine is frequently alkylated in DNA. The SOS-induced polymerases have been shown to be capable of bypassing various DNA damage products in <i>Escherichia coli</i>. Herein, we explored the influences of four <i>N</i><sup>2</sup>-alkyl-dG lesions (alkyl = ethyl, <i>n</i>-butyl, isobutyl, or <i>sec</i>-butyl) on DNA replication in AB1157 <i>E. coli</i> cells and the corresponding strains with polymerases (Pol) II, IV, and V being individually or simultaneously knocked out. We found that <i>N</i><sup>2</sup>-Et-dG is slightly less blocking to DNA replication than the <i>N</i><sup>2</sup>-Bu-dG lesions, which display very similar replication bypass efficiencies. Additionally, Pol II and, to a lesser degree,"],"journal":["Chemical research in toxicology"],"pubmed_title":["DNA Polymerase II Supports the Replicative Bypass of <i>N</i><sup>2</sup>-Alkyl-2'-deoxyguanosine Lesions in <i>Escherichia coli</i> Cells."],"pmcid":["PMC7965357"],"funding_grant_id":["R35 ES031707"],"pubmed_authors":["Wu J","Wang Y"],"additional_accession":[]},"is_claimable":false,"name":"DNA Polymerase II Supports the Replicative Bypass of <i>N</i><sup>2</sup>-Alkyl-2'-deoxyguanosine Lesions in <i>Escherichia coli</i> Cells.","description":"Alkylation represents a main form of DNA damage. The <i>N</i><sup>2</sup> position of guanine is frequently alkylated in DNA. The SOS-induced polymerases have been shown to be capable of bypassing various DNA damage products in <i>Escherichia coli</i>. Herein, we explored the influences of four <i>N</i><sup>2</sup>-alkyl-dG lesions (alkyl = ethyl, <i>n</i>-butyl, isobutyl, or <i>sec</i>-butyl) on DNA replication in AB1157 <i>E. coli</i> cells and the corresponding strains with polymerases (Pol) II, IV, and V being individually or simultaneously knocked out. We found that <i>N</i><sup>2</sup>-Et-dG is slightly less blocking to DNA replication than the <i>N</i><sup>2</sup>-Bu-dG lesions, which display very similar replication bypass efficiencies. Additionally, Pol II and, to a lesser degree,","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2025-04-18T15:11:05.502Z","creation":"2025-02-18T23:35:40.279Z"},"accession":"S-EPMC7965357","cross_references":{"pubmed":["33417436"],"doi":["10.1021/acs.chemrestox.0c00478"]}}