biostudies-literatureUnknown12Cunningham CC<h4>Background</h4>MicroRNAs (miRNAs) are small non-coding RNAs which have been implicated as potential biomarkers or therapeutic targets in autoimmune diseases. This study examines circulatory miRNAs in RA patients and further investigates if a serum miRNA signature precedes clinical manifestations of disease in arthralgia or "at-risk individuals".<h4>Methods</h4>Serum was collected from HC subjects (N = 20), RA patients (N = 50), and arthralgia subjects (N = 10), in addition to a subgroup of the RA patients post-methotrexate (MTX) (N = 18). The FirePlex miRNA Immunology-V2 panel was selected for multiplex analysis of 68 miRNAs in each sample. DNA intelligent analysis (DIANA)-mirPath and Ingenuity Pathway Analysis (IPA) software were used to predict pathways targeted by the dysregulated mFrontiers in immunology633201https://www.ebi.ac.uk/biostudies/studies/S-EPMC7966707biostudies-literatureSerum miRNA Signature in Rheumatoid Arthritis and "At-Risk Individuals".PMC7966707Wade SFloudas ACregan SCunningham CCMcGarry TVeale DJOrr CFearon UfalseSerum miRNA Signature in Rheumatoid Arthritis and "At-Risk Individuals".<h4>Background</h4>MicroRNAs (miRNAs) are small non-coding RNAs which have been implicated as potential biomarkers or therapeutic targets in autoimmune diseases. This study examines circulatory miRNAs in RA patients and further investigates if a serum miRNA signature precedes clinical manifestations of disease in arthralgia or "at-risk individuals".<h4>Methods</h4>Serum was collected from HC subjects (N = 20), RA patients (N = 50), and arthralgia subjects (N = 10), in addition to a subgroup of the RA patients post-methotrexate (MTX) (N = 18). The FirePlex miRNA Immunology-V2 panel was selected for multiplex analysis of 68 miRNAs in each sample. DNA intelligent analysis (DIANA)-mirPath and Ingenuity Pathway Analysis (IPA) software were used to predict pathways targeted by the dysregulated m2021-01-01T00:00:00Z20212025-04-18T16:39:07.56Z2025-04-07T03:58:40.689ZS-EPMC79667073374697110.3389/fimmu.2021.633201