{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["35"],"submitter":["Nguyen HV"],"funding":["New Zealand Ministry of Business Innovation and Employment"],"pubmed_abstract":["Cytosine-phosphate-guanine (CpG) oligonucleotides are commonly-used vaccine adjuvants to promote the activation of antigen-presenting cells (APCs). To mount an effective immune response, CpG needs to be internalized and bind to its endosomal Toll-like receptor 9 (TLR-9) inside the APCs. Using flow cytometry and fluorescence microscopy, this article presents the cellular uptake data of the amino-dextran nanoparticle (aDNP) and aDNP loaded with CpG immobilized on its surface by either electrostatic adsorption or covalent conjugation. The uptake of fluorescently-labelled aDNPs by murine splenic dendritic cells and macrophages was determined by flow cytometry and uptake by murine bone-marrow-derived dendritic cells was evaluated by fluorescence microscopy. The data presented in this paper corr"],"journal":["Data in brief"],"pagination":["106883"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7966978"],"repository":["biostudies-literature"],"pubmed_title":["Data on the uptake of CpG-loaded amino-dextran nanoparticles by antigen-presenting cells."],"pmcid":["PMC7966978"],"pubmed_authors":["Nguyen HV","Young SL","Campbell K","Painter GF","Walker GF"],"additional_accession":[]},"is_claimable":false,"name":"Data on the uptake of CpG-loaded amino-dextran nanoparticles by antigen-presenting cells.","description":"Cytosine-phosphate-guanine (CpG) oligonucleotides are commonly-used vaccine adjuvants to promote the activation of antigen-presenting cells (APCs). To mount an effective immune response, CpG needs to be internalized and bind to its endosomal Toll-like receptor 9 (TLR-9) inside the APCs. Using flow cytometry and fluorescence microscopy, this article presents the cellular uptake data of the amino-dextran nanoparticle (aDNP) and aDNP loaded with CpG immobilized on its surface by either electrostatic adsorption or covalent conjugation. The uptake of fluorescently-labelled aDNPs by murine splenic dendritic cells and macrophages was determined by flow cytometry and uptake by murine bone-marrow-derived dendritic cells was evaluated by fluorescence microscopy. The data presented in this paper corr","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Apr","modification":"2025-04-22T08:17:02.181Z","creation":"2025-04-05T22:29:04.401Z"},"accession":"S-EPMC7966978","cross_references":{"pubmed":["33748357"],"doi":["10.1016/j.dib.2021.106883"]}}