biostudies-literatureUnknown35Nguyen HVNew Zealand Ministry of Business Innovation and EmploymentCytosine-phosphate-guanine (CpG) oligonucleotides are commonly-used vaccine adjuvants to promote the activation of antigen-presenting cells (APCs). To mount an effective immune response, CpG needs to be internalized and bind to its endosomal Toll-like receptor 9 (TLR-9) inside the APCs. Using flow cytometry and fluorescence microscopy, this article presents the cellular uptake data of the amino-dextran nanoparticle (aDNP) and aDNP loaded with CpG immobilized on its surface by either electrostatic adsorption or covalent conjugation. The uptake of fluorescently-labelled aDNPs by murine splenic dendritic cells and macrophages was determined by flow cytometry and uptake by murine bone-marrow-derived dendritic cells was evaluated by fluorescence microscopy. The data presented in this paper corrData in brief106883https://www.ebi.ac.uk/biostudies/studies/S-EPMC7966978biostudies-literatureData on the uptake of CpG-loaded amino-dextran nanoparticles by antigen-presenting cells.PMC7966978Nguyen HVYoung SLCampbell KPainter GFWalker GFfalseData on the uptake of CpG-loaded amino-dextran nanoparticles by antigen-presenting cells.Cytosine-phosphate-guanine (CpG) oligonucleotides are commonly-used vaccine adjuvants to promote the activation of antigen-presenting cells (APCs). To mount an effective immune response, CpG needs to be internalized and bind to its endosomal Toll-like receptor 9 (TLR-9) inside the APCs. Using flow cytometry and fluorescence microscopy, this article presents the cellular uptake data of the amino-dextran nanoparticle (aDNP) and aDNP loaded with CpG immobilized on its surface by either electrostatic adsorption or covalent conjugation. The uptake of fluorescently-labelled aDNPs by murine splenic dendritic cells and macrophages was determined by flow cytometry and uptake by murine bone-marrow-derived dendritic cells was evaluated by fluorescence microscopy. The data presented in this paper corr2021-01-01T00:00:00Z2021 Apr2025-04-22T08:17:02.181Z2025-04-05T22:29:04.401ZS-EPMC79669783374835710.1016/j.dib.2021.106883