{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["24(3)"],"submitter":["Du Z"],"funding":["China Postdoctoral Science Foundation"],"pubmed_abstract":["Human milk oligosaccharides (HMOs), the third most abundant solid component of human milk, are reported to be beneficial to infant health. The biosynthesis of lacto-<i>N</i>-biose (LNB), the building block for HMOs, suffers from excessive addition of cofactors and intermediate inhibition. Here, we developed an <i>in vitro</i> multienzyme cascade composed of LNB module, ATP regeneration, and pyruvate oxidase-driven phosphate recycling to produce LNB. The integration between ATP regeneration and Pi alleviation increased the LNB conversion ratio and resulted in a ΔG'° decrease of 540 KJ/mol. Under optimal conditions, the LNB conversion ratio was improved from 0.34 to 0.83 mol/mol GlcNAc and the ATP addition decreased to 50%. Finally, 0.96 mol/mol GlcNAc and 71.6 mg LNB g<sup>-1</sup> GlcNAc h<sup>-1</sup> of LNB yield was achieved in a 100-mL reaction system. The synergistic strategy not only paves the way for producing LNB but also facilitates other chemicals with multienzyme cascades."],"journal":["iScience"],"pagination":["102236"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7967015"],"repository":["biostudies-literature"],"pubmed_title":["Lacto-<i>N</i>-biose synthesis via a modular enzymatic cascade with ATP regeneration."],"pmcid":["PMC7967015"],"pubmed_authors":["Fang X","Liu Z","Tan Y","Guo W","Jia Y","Niu K","Du Z"],"additional_accession":[]},"is_claimable":false,"name":"Lacto-<i>N</i>-biose synthesis via a modular enzymatic cascade with ATP regeneration.","description":"Human milk oligosaccharides (HMOs), the third most abundant solid component of human milk, are reported to be beneficial to infant health. The biosynthesis of lacto-<i>N</i>-biose (LNB), the building block for HMOs, suffers from excessive addition of cofactors and intermediate inhibition. Here, we developed an <i>in vitro</i> multienzyme cascade composed of LNB module, ATP regeneration, and pyruvate oxidase-driven phosphate recycling to produce LNB. The integration between ATP regeneration and Pi alleviation increased the LNB conversion ratio and resulted in a ΔG'° decrease of 540 KJ/mol. Under optimal conditions, the LNB conversion ratio was improved from 0.34 to 0.83 mol/mol GlcNAc and the ATP addition decreased to 50%. Finally, 0.96 mol/mol GlcNAc and 71.6 mg LNB g<sup>-1</sup> GlcNAc h<sup>-1</sup> of LNB yield was achieved in a 100-mL reaction system. The synergistic strategy not only paves the way for producing LNB but also facilitates other chemicals with multienzyme cascades.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2026-04-16T15:03:43.628Z","creation":"2025-02-19T02:14:11.388Z"},"accession":"S-EPMC7967015","cross_references":{"pubmed":["33748718"],"doi":["10.1016/j.isci.2021.102236"]}}