{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Aroonsri A"],"funding":["National Science and Technology Development Agency (TH)","Thailand Research Fund","National Science and Technology Development Agency"],"pagination":["20"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7967978"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(1)"],"pubmed_abstract":["<h4>Background</h4>Bioinformatic genome surveys indicate that self-cleaving ribonucleic acids (ribozymes) appear to be widespread among all domains of life, although the functions of only a small number have been validated by biochemical methods. Alternatively, cell-based reporter gene assays can be used to validate ribozyme function. However, reporter activity can be confounded by phenomena unrelated to ribozyme-mediated cleavage of RNA.<h4>Results</h4>We established a ribozyme reporter system in Escherichia coli in which a significant reduction of reporter activity is manifest when an active ribozyme sequence is fused to the reporter gene and the expression of a foreign Bacillus subtilis RNaseJ1 5' exonuclease is induced from a chromosomally-integrated gene in the same cell.<h4>Conclusio"],"journal":["BMC molecular and cell biology"],"pubmed_title":["A cell-based ribozyme reporter system employing a chromosomally-integrated 5' exonuclease gene."],"pmcid":["PMC7967978"],"funding_grant_id":["P17-51076","TRG608001"],"pubmed_authors":["Kongsee J","Shaw PJ","Aubry DA","Aroonsri A","Gunawan JD"],"additional_accession":[]},"is_claimable":false,"name":"A cell-based ribozyme reporter system employing a chromosomally-integrated 5' exonuclease gene.","description":"<h4>Background</h4>Bioinformatic genome surveys indicate that self-cleaving ribonucleic acids (ribozymes) appear to be widespread among all domains of life, although the functions of only a small number have been validated by biochemical methods. Alternatively, cell-based reporter gene assays can be used to validate ribozyme function. However, reporter activity can be confounded by phenomena unrelated to ribozyme-mediated cleavage of RNA.<h4>Results</h4>We established a ribozyme reporter system in Escherichia coli in which a significant reduction of reporter activity is manifest when an active ribozyme sequence is fused to the reporter gene and the expression of a foreign Bacillus subtilis RNaseJ1 5' exonuclease is induced from a chromosomally-integrated gene in the same cell.<h4>Conclusio","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2025-04-05T13:48:26.011Z","creation":"2024-12-04T06:32:08.833Z"},"accession":"S-EPMC7967978","cross_references":{"pubmed":["33726662"],"doi":["10.1186/s12860-021-00357-7"]}}