biostudies-literatureAroonsri ANational Science and Technology Development Agency (TH)Thailand Research FundNational Science and Technology Development Agency20https://www.ebi.ac.uk/biostudies/studies/S-EPMC7967978biostudies-literatureUnknown22(1)<h4>Background</h4>Bioinformatic genome surveys indicate that self-cleaving ribonucleic acids (ribozymes) appear to be widespread among all domains of life, although the functions of only a small number have been validated by biochemical methods. Alternatively, cell-based reporter gene assays can be used to validate ribozyme function. However, reporter activity can be confounded by phenomena unrelated to ribozyme-mediated cleavage of RNA.<h4>Results</h4>We established a ribozyme reporter system in Escherichia coli in which a significant reduction of reporter activity is manifest when an active ribozyme sequence is fused to the reporter gene and the expression of a foreign Bacillus subtilis RNaseJ1 5' exonuclease is induced from a chromosomally-integrated gene in the same cell.<h4>ConclusioBMC molecular and cell biologyA cell-based ribozyme reporter system employing a chromosomally-integrated 5' exonuclease gene.PMC7967978P17-51076TRG608001Kongsee JShaw PJAubry DAAroonsri AGunawan JDfalseA cell-based ribozyme reporter system employing a chromosomally-integrated 5' exonuclease gene.<h4>Background</h4>Bioinformatic genome surveys indicate that self-cleaving ribonucleic acids (ribozymes) appear to be widespread among all domains of life, although the functions of only a small number have been validated by biochemical methods. Alternatively, cell-based reporter gene assays can be used to validate ribozyme function. However, reporter activity can be confounded by phenomena unrelated to ribozyme-mediated cleavage of RNA.<h4>Results</h4>We established a ribozyme reporter system in Escherichia coli in which a significant reduction of reporter activity is manifest when an active ribozyme sequence is fused to the reporter gene and the expression of a foreign Bacillus subtilis RNaseJ1 5' exonuclease is induced from a chromosomally-integrated gene in the same cell.<h4>Conclusio2021-01-01T00:00:00Z2021 Mar2025-04-05T13:48:26.011Z2024-12-04T06:32:08.833ZS-EPMC79679783372666210.1186/s12860-021-00357-7