biostudies-literatureCheng YHMinistry of Science and Technology, TaiwanChina Medical UniversityShow Chwan Memorial Hospitale0247550https://www.ebi.ac.uk/biostudies/studies/S-EPMC7968633biostudies-literatureUnknown16(3)Human pancreatic ductal adenocarcinoma (PDAC) is a deadly cancer type with a very high mortality rate. Inflammatory cytokine such as tumor necrosis factor- alpha (TNF-α) plays a pivotal role in the progression of PDAC. Recently, suppression of cell invasion by preventive agents has received considerable attention in the prevention of metastatic tumors. Several clinical studies suggested that natural forms or analogues of fat-soluble vitamins such as vitamin A and vitamin D can work as anti-cancer agents to inhibit the development of cancer. In this study, our results demonstrated that co-treatment of 13-cis retinoic acid (13-cis RA) and 1,25-dihydroxyvitamin D3 (1,25-VD3) significantly inhibited TNF-α mediated cell invasion in PDAC in vitro. Cotreatment of 13-cis RA and 1,25-VD3 also inhibPloS oneTreatment of 13-cis retinoic acid and 1,25-dihydroxyvitamin D3 inhibits TNF-alpha-mediated expression of MMP-9 protein and cell invasion through the suppression of JNK pathway and microRNA 221 in human pancreatic adenocarcinoma cancer cells.PMC7968633MOST-104-2320-B-039-041-MY3,107-2320-B-039-008-MY3, 103-2811-B-039-009, 105-2811-B-039-005,105-2811-B-039-031, 106-2811-B-039- 002, 106-2811-B-039-016, MOST 107-2320-B-005-003 -MY3, 107-2621-M-005-008 -MY3, 108-2321-B-005-004CMU102-ASIA-23, CMU103-ASIA-20, CMU103-S-46, CMU104-S-32SRD-109047, SRD-109048Chao CYTsai SYTang FYSyu JNLin CCYang MDLin HYCheng YHChiang EIfalseTreatment of 13-cis retinoic acid and 1,25-dihydroxyvitamin D3 inhibits TNF-alpha-mediated expression of MMP-9 protein and cell invasion through the suppression of JNK pathway and microRNA 221 in human pancreatic adenocarcinoma cancer cells.Human pancreatic ductal adenocarcinoma (PDAC) is a deadly cancer type with a very high mortality rate. Inflammatory cytokine such as tumor necrosis factor- alpha (TNF-α) plays a pivotal role in the progression of PDAC. Recently, suppression of cell invasion by preventive agents has received considerable attention in the prevention of metastatic tumors. Several clinical studies suggested that natural forms or analogues of fat-soluble vitamins such as vitamin A and vitamin D can work as anti-cancer agents to inhibit the development of cancer. In this study, our results demonstrated that co-treatment of 13-cis retinoic acid (13-cis RA) and 1,25-dihydroxyvitamin D3 (1,25-VD3) significantly inhibited TNF-α mediated cell invasion in PDAC in vitro. Cotreatment of 13-cis RA and 1,25-VD3 also inhib2021-01-01T00:00:00Z20212025-04-21T17:07:21.897Z2022-02-09T10:17:10.431ZS-EPMC79686333373007210.1371/journal.pone.0247550