{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Venkatramanan S"],"funding":["Rutgers University","National Institutes of Health","NIGMS NIH HHS"],"pagination":["jcs247866"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7970510"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["134(6)"],"pubmed_abstract":["Hippo signaling mediates influences of cytoskeletal tension on organ growth. TRIP6 and LIMD1 have each been identified as being required for tension-dependent inhibition of the Hippo pathway LATS kinases and their recruitment to adherens junctions, but the relationship between TRIP6 and LIMD1 was unknown. Using siRNA-mediated gene knockdown, we show that TRIP6 is required for LIMD1 localization to adherens junctions, whereas LIMD1 is not required for TRIP6 localization. TRIP6, but not LIMD1, is also required for the recruitment of vinculin and VASP to adherens junctions. Knockdown of TRIP6 or vinculin, but not of LIMD1, also influences the localization of myosin and F-actin. In TRIP6 knockdown cells, actin stress fibers are lost apically but increased basally, and there is a corresponding "],"journal":["Journal of cell science"],"pubmed_title":["TRIP6 is required for tension at adherens junctions."],"pmcid":["PMC7970510"],"funding_grant_id":["R35 GM131748","GM131748"],"pubmed_authors":["Irvine KD","Ibar C","Venkatramanan S"],"additional_accession":[]},"is_claimable":false,"name":"TRIP6 is required for tension at adherens junctions.","description":"Hippo signaling mediates influences of cytoskeletal tension on organ growth. TRIP6 and LIMD1 have each been identified as being required for tension-dependent inhibition of the Hippo pathway LATS kinases and their recruitment to adherens junctions, but the relationship between TRIP6 and LIMD1 was unknown. Using siRNA-mediated gene knockdown, we show that TRIP6 is required for LIMD1 localization to adherens junctions, whereas LIMD1 is not required for TRIP6 localization. TRIP6, but not LIMD1, is also required for the recruitment of vinculin and VASP to adherens junctions. Knockdown of TRIP6 or vinculin, but not of LIMD1, also influences the localization of myosin and F-actin. In TRIP6 knockdown cells, actin stress fibers are lost apically but increased basally, and there is a corresponding ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2026-07-16T12:01:53.883Z","creation":"2025-04-04T19:54:12.717Z"},"accession":"S-EPMC7970510","cross_references":{"pubmed":["33558314"],"doi":["10.1242/jcs.247866"]}}