{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Turnsek J"],"funding":["NIEHS NIH HHS","Biological and Environmental Research","Gordon and Betty Moore Foundation","National Institutes of Health","Czech Science Foundation","NIH HHS","NIGMS NIH HHS","National Science Foundation","European Regional Development Fund"],"pagination":["e52770"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7972479"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10"],"pubmed_abstract":["Iron is a biochemically critical metal cofactor in enzymes involved in photosynthesis, cellular respiration, nitrate assimilation, nitrogen fixation, and reactive oxygen species defense. Marine microeukaryotes have evolved a phytotransferrin-based iron uptake system to cope with iron scarcity, a major factor limiting primary productivity in the global ocean. Diatom phytotransferrin is endocytosed; however, proteins downstream of this environmentally ubiquitous iron receptor are unknown. We applied engineered ascorbate peroxidase APEX2-based subcellular proteomics to catalog proximal proteins of phytotransferrin in the model marine diatom <i>Phaeodactylum tricornutum</i>. Proteins encoded by poorly characterized iron-sensitive genes were identified including three that are expressed from a "],"journal":["eLife"],"pubmed_title":["Proximity proteomics in a marine diatom reveals a putative cell surface-to-chloroplast iron trafficking pathway."],"pmcid":["PMC7972479"],"funding_grant_id":["GBMF5006","F31 1F31ES030613-01","NSF-OCE-1756884","NSF-MCB-1818390","F31 ES030613","GBMF3828","CZ.02.1.01/0.0/0.0/16_019/0000759","GBMF4958","DE-SC0018344","R24 GM137200","1F31ES030613-01","21-03224S"],"pubmed_authors":["Allen AE","Bielinski VA","Brunson JK","Viedma MDPM","Horak A","Obornik M","Turnsek J","Deerinck TJ"],"additional_accession":[]},"is_claimable":false,"name":"Proximity proteomics in a marine diatom reveals a putative cell surface-to-chloroplast iron trafficking pathway.","description":"Iron is a biochemically critical metal cofactor in enzymes involved in photosynthesis, cellular respiration, nitrate assimilation, nitrogen fixation, and reactive oxygen species defense. Marine microeukaryotes have evolved a phytotransferrin-based iron uptake system to cope with iron scarcity, a major factor limiting primary productivity in the global ocean. Diatom phytotransferrin is endocytosed; however, proteins downstream of this environmentally ubiquitous iron receptor are unknown. We applied engineered ascorbate peroxidase APEX2-based subcellular proteomics to catalog proximal proteins of phytotransferrin in the model marine diatom <i>Phaeodactylum tricornutum</i>. Proteins encoded by poorly characterized iron-sensitive genes were identified including three that are expressed from a ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Feb","modification":"2026-04-13T03:12:40.562Z","creation":"2025-02-19T02:12:53.86Z"},"accession":"S-EPMC7972479","cross_references":{"pubmed":["33591270"],"doi":["10.7554/eLife.52770"]}}