biostudies-literatureSingh KBritish Heart FoundationWellcome Trust640837https://www.ebi.ac.uk/biostudies/studies/S-EPMC7973371biostudies-literatureUnknown12Inflammatory cardiomyopathy covers a group of diseases characterized by inflammation and dysfunction of the heart muscle. The immunosuppressive agents such as prednisolone, azathioprine and cyclosporine are modestly effective treatments, but a molecular rationale underpinning such therapy or the development of new therapeutic strategies is lacking. We aimed to develop a network-based approach to identify therapeutic targets for inflammatory cardiomyopathy from the evolving myocardial transcriptome in a mouse model of the disease. We performed bulk RNA sequencing of hearts at early, mid and late time points from mice with experimental autoimmune myocarditis. We identified a cascade of pathway-level events involving early activation of cytokine and chemokine-signaling pathways that precede lFrontiers in immunologyTranscriptomic Analysis of Inflammatory Cardiomyopathy Identifies Molecular Signatures of Disease and Informs <i>in silico</i> Prediction of a Network-Based Rationale for Therapy.PMC7973371RG/18/1/33351203141/Z/16/Z204969/Z/16/ZCH/09/003/26631PG/16/100/32632RE/13/1/30181090532/Z/09/ZFang HWright BLockstone HWilliams ROKnight JCSingh KBhattacharya SCihakova DDavies GfalseTranscriptomic Analysis of Inflammatory Cardiomyopathy Identifies Molecular Signatures of Disease and Informs <i>in silico</i> Prediction of a Network-Based Rationale for Therapy.Inflammatory cardiomyopathy covers a group of diseases characterized by inflammation and dysfunction of the heart muscle. The immunosuppressive agents such as prednisolone, azathioprine and cyclosporine are modestly effective treatments, but a molecular rationale underpinning such therapy or the development of new therapeutic strategies is lacking. We aimed to develop a network-based approach to identify therapeutic targets for inflammatory cardiomyopathy from the evolving myocardial transcriptome in a mouse model of the disease. We performed bulk RNA sequencing of hearts at early, mid and late time points from mice with experimental autoimmune myocarditis. We identified a cascade of pathway-level events involving early activation of cytokine and chemokine-signaling pathways that precede l2021-01-01T00:00:00Z20212026-05-01T17:34:55.242Z2024-11-21T10:23:53.377ZS-EPMC79733713374698310.3389/fimmu.2021.640837