biostudies-literatureTeertam SKDepartment of Biotechnology , Ministry of Science and TechnologyDepartment of Science and Technology, Ministry of Science and Technology, IndiaDepartment of Science and Technology, Ministry of Science and Technology6339https://www.ebi.ac.uk/biostudies/studies/S-EPMC7973546biostudies-literatureUnknown11(1)Cerebral ischemia (CI) is a severe cause of neurological dysfunction and mortality. Sirtuin-1 (Silent information regulator family protein 1, SIRT1), an oxidized nicotinamide adenine dinucleotide (NAD⁺)-dependent protein deacetylase, plays an important role in protection against several neurodegenerative disorders. The present study aims to investigate the protective role of SIRT1 after CI in experimental young and aged rats and humans. Also, the study examines the possible regulatory mechanisms of neuronal death in CI settings. Immunoblotting and immunohistochemistry were used to evaluate changes in the expression of SIRT1, JNK/ERK/MAPK/AKT signaling, and pro-apoptotic caspase-3 in experimental rats and CI patients. The study findings demonstrated that, in aged experimental ratScientific reportsDifferential role of SIRT1/MAPK pathway during cerebral ischemia in rats and humans.PMC7973546DBT/04/0401/2017/01181CRG/2020/005021SR/CSRI/196/2016Teertam SKPrakash Babu PfalseDifferential role of SIRT1/MAPK pathway during cerebral ischemia in rats and humans.Cerebral ischemia (CI) is a severe cause of neurological dysfunction and mortality. Sirtuin-1 (Silent information regulator family protein 1, SIRT1), an oxidized nicotinamide adenine dinucleotide (NAD⁺)-dependent protein deacetylase, plays an important role in protection against several neurodegenerative disorders. The present study aims to investigate the protective role of SIRT1 after CI in experimental young and aged rats and humans. Also, the study examines the possible regulatory mechanisms of neuronal death in CI settings. Immunoblotting and immunohistochemistry were used to evaluate changes in the expression of SIRT1, JNK/ERK/MAPK/AKT signaling, and pro-apoptotic caspase-3 in experimental rats and CI patients. The study findings demonstrated that, in aged experimental rat2021-01-01T00:00:00Z2021 Mar2025-04-18T13:27:35.924Z2025-04-06T23:13:20.686ZS-EPMC79735463373756010.1038/s41598-021-85577-9