biostudies-literatureBo FShandong Modern Agricultural Technology and Industry SystemShandong Agricultural Science and Technology Fund Program- Park Industry Upgrading ProjectNational Key Research and Development Program of China6327https://www.ebi.ac.uk/biostudies/studies/S-EPMC7973580biostudies-literatureUnknown11(1)Lysine 2-hydroxyisobutyrylation (Khib) is a novel naturally occurring post-translational modification. The system Khib identification at proteomics level has been performed in various species and tissues to characterize the role of Khib in biological activities. However, the study of Khib in plant species is relatively less. In the present study, the first plant root tissues lysine 2-hydroxyisobutyrylome analysis was performed in wheat with antibody immunoprecipitation affinity, high resolution mass spectrometry-based proteomics and bioinformatics analysis. In total, 6328 Khib sites in 2186 proteins were repeatedly identified in three replicates. These Khib proteins showed a wide subcellular location distribution. Function and pathways characterization of these Khib proteins indicated thatScientific reportsGlobal analysis of lysine 2-hydroxyisobutyrylation in wheat root.PMC79735802018YFD03006012019YQ001SDAIT-01-06Zongshuai WGeng LLing'an KShengdong LFang CBo FZheng WChunhua GfalseGlobal analysis of lysine 2-hydroxyisobutyrylation in wheat root.Lysine 2-hydroxyisobutyrylation (Khib) is a novel naturally occurring post-translational modification. The system Khib identification at proteomics level has been performed in various species and tissues to characterize the role of Khib in biological activities. However, the study of Khib in plant species is relatively less. In the present study, the first plant root tissues lysine 2-hydroxyisobutyrylome analysis was performed in wheat with antibody immunoprecipitation affinity, high resolution mass spectrometry-based proteomics and bioinformatics analysis. In total, 6328 Khib sites in 2186 proteins were repeatedly identified in three replicates. These Khib proteins showed a wide subcellular location distribution. Function and pathways characterization of these Khib proteins indicated that2021-01-01T00:00:00Z2021 Mar2026-04-07T19:18:50.205Z2025-04-06T13:14:39.795ZS-EPMC79735803373771910.1038/s41598-021-85879-y