biostudies-literatureKang HIKorea Forest Service6359https://www.ebi.ac.uk/biostudies/studies/S-EPMC7973583biostudies-literatureUnknown11(1)Tilia species are valuable woody species due to their beautiful shape and role as honey trees. Somatic embryogenesis can be an alternative method for mass propagation of T. amurensis. However, the molecular mechanisms of T. amurensis somatic embryogenesis are yet to be known. Here, we conducted comparative transcriptional analysis during somatic embryogenesis of T. amurensis. RNA-Seq identified 1505 differentially expressed genes, including developmental regulatory genes. Auxin related genes such as YUC, AUX/IAA and ARF and signal transduction pathway related genes including LEA and SERK were differentially regulated during somatic embryogenesis. Also, B3 domain family (LEC2, FUS3), VAL and PKL, the regulatory transcription factors, were differentially expressed by somatic embryo developmeScientific reportsComparative transcriptome analysis during developmental stages of direct somatic embryogenesis in Tilia amurensis Rupr.PMC7973583FTIS 2020185D10-2022-AA02Shim DKang HIKwon SHPark JMLee CBKang KSfalseComparative transcriptome analysis during developmental stages of direct somatic embryogenesis in Tilia amurensis Rupr.Tilia species are valuable woody species due to their beautiful shape and role as honey trees. Somatic embryogenesis can be an alternative method for mass propagation of T. amurensis. However, the molecular mechanisms of T. amurensis somatic embryogenesis are yet to be known. Here, we conducted comparative transcriptional analysis during somatic embryogenesis of T. amurensis. RNA-Seq identified 1505 differentially expressed genes, including developmental regulatory genes. Auxin related genes such as YUC, AUX/IAA and ARF and signal transduction pathway related genes including LEA and SERK were differentially regulated during somatic embryogenesis. Also, B3 domain family (LEC2, FUS3), VAL and PKL, the regulatory transcription factors, were differentially expressed by somatic embryo developme2021-01-01T00:00:00Z2021 Mar2025-04-21T17:08:26.414Z2025-04-21T17:08:26.414ZS-EPMC79735833373767310.1038/s41598-021-85886-z