biostudies-literatureStrobbia PNIBIB NIH HHS4090-4102https://www.ebi.ac.uk/biostudies/studies/S-EPMC7977455biostudies-literatureUnknown11(9)For the majority of cancer patients, surgery is the primary method of treatment. In these cases, accurately removing the entire tumor without harming surrounding tissue is critical; however, due to the lack of intraoperative imaging techniques, surgeons rely on visual and physical inspection to identify tumors. Surface-enhanced Raman scattering (SERS) is emerging as a non-invasive optical alternative for intraoperative tumor identification, with high accuracy and stability. However, Raman detection requires dark rooms to work, which is not consistent with surgical settings. <b>Methods:</b> Herein, we used SERS nanoprobes combined with shifted-excitation Raman difference spectroscopy (SERDS) detection, to accurately detect tumors in xenograft murine model. <b>Results:</b> We demonstrate forTheranosticsAccurate <i>in vivo</i> tumor detection using plasmonic-enhanced shifted-excitation Raman difference spectroscopy (SERDS).PMC7977455R01 EB028078Liu YFales AMPfefer TJSumpf BCupil-Garcia VCrawford BMStrobbia PMaiwald MVo-Dinh TfalseAccurate <i>in vivo</i> tumor detection using plasmonic-enhanced shifted-excitation Raman difference spectroscopy (SERDS).For the majority of cancer patients, surgery is the primary method of treatment. In these cases, accurately removing the entire tumor without harming surrounding tissue is critical; however, due to the lack of intraoperative imaging techniques, surgeons rely on visual and physical inspection to identify tumors. Surface-enhanced Raman scattering (SERS) is emerging as a non-invasive optical alternative for intraoperative tumor identification, with high accuracy and stability. However, Raman detection requires dark rooms to work, which is not consistent with surgical settings. <b>Methods:</b> Herein, we used SERS nanoprobes combined with shifted-excitation Raman difference spectroscopy (SERDS) detection, to accurately detect tumors in xenograft murine model. <b>Results:</b> We demonstrate for2021-01-01T00:00:00Z20212025-04-25T18:38:41.406Z2025-04-06T07:41:18.14ZS-EPMC79774553375405010.7150/thno.53101