biostudies-literatureUnknown9Zang LOsteoporosis is the most common aging-associated bone disease and is caused by hyperactivation of osteoclastic activity. We previously reported that the hexane extract of ginger rhizome [ginger hexane extract (GHE)] could suppress receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastogenesis in RAW264.7 cells. However, the anti-osteoclastic components in GHE have not yet been identified. In this study, we separated GHE into several fractions using silica gel column chromatography and evaluated their effects on osteoclastogenesis using a RAW264.7 cell osteoclast differentiation assay (<i>in vitro</i>) and the zebrafish scale model of osteoporosis (<i>in vivo</i>). We identified that the fractions containing 10-gingerol suppressed osteoclastogenesis in RAW264.7 cellsFrontiers in cell and developmental biology588093https://www.ebi.ac.uk/biostudies/studies/S-EPMC7978033biostudies-literature10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales.PMC7978033Nakayama HShimada YZang LKatsuzaki HNishimura NKagotani KHayashi ABhagat JFujimoto YSono Rfalse10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales.Osteoporosis is the most common aging-associated bone disease and is caused by hyperactivation of osteoclastic activity. We previously reported that the hexane extract of ginger rhizome [ginger hexane extract (GHE)] could suppress receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastogenesis in RAW264.7 cells. However, the anti-osteoclastic components in GHE have not yet been identified. In this study, we separated GHE into several fractions using silica gel column chromatography and evaluated their effects on osteoclastogenesis using a RAW264.7 cell osteoclast differentiation assay (<i>in vitro</i>) and the zebrafish scale model of osteoporosis (<i>in vivo</i>). We identified that the fractions containing 10-gingerol suppressed osteoclastogenesis in RAW264.7 cells2021-01-01T00:00:00Z20212026-07-16T10:09:56.542Z2025-04-06T23:14:29.592ZS-EPMC79780333374810010.3389/fcell.2021.588093