{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(1)"],"submitter":["Akuta T"],"pubmed_abstract":["Scabies is a highly contagious skin disease caused by the mite Sarcoptes scabiei that affects many mammals. However, the sensitivity of traditional tests for scabies diagnosis in humans is less than 50%. To simplify the diagnosis of scabies, methods that are simple, sensitive, specific, and cost-effective are required. We developed an immunodiagnostic test based on S. scabiei var. nyctereutis RNA-seq data collected from Japanese raccoon dogs with sarcoptic mange. Three candidate antigens-a highly expressed hypothetical protein \"QR98_0091190,\" another mite allergen known as \"SMIPP-Cc,\" and an abundant \"vitellogenin-like protein\"-were evaluated by western-blot analysis. A lateral flow immunoassay, using specific antibodies against the vitellogenin-like protein, successfully detected scabies "],"journal":["Scientific reports"],"pagination":["6455"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7979781"],"repository":["biostudies-literature"],"pubmed_title":["Development of a rapid scabies immunodiagnostic assay based on transcriptomic analysis of Sarcoptes scabiei var. nyctereutis."],"pmcid":["PMC7979781"],"pubmed_authors":["Masataka","Kido N","Hikosaka K","Ono Y","Imaizumi K","Tachibana SI","Hori F","Omiya T","Kawahara M","Ueda T","Tarui H","Nakajima Y","Oouchi Y","Morikaku K","Tanaka S","Nakaoka S","Sakuma C","Minegishi D","Nakagawa","Kikuchi-Ueda T","Akuta T","Tada Y"],"additional_accession":[]},"is_claimable":false,"name":"Development of a rapid scabies immunodiagnostic assay based on transcriptomic analysis of Sarcoptes scabiei var. nyctereutis.","description":"Scabies is a highly contagious skin disease caused by the mite Sarcoptes scabiei that affects many mammals. However, the sensitivity of traditional tests for scabies diagnosis in humans is less than 50%. To simplify the diagnosis of scabies, methods that are simple, sensitive, specific, and cost-effective are required. We developed an immunodiagnostic test based on S. scabiei var. nyctereutis RNA-seq data collected from Japanese raccoon dogs with sarcoptic mange. Three candidate antigens-a highly expressed hypothetical protein \"QR98_0091190,\" another mite allergen known as \"SMIPP-Cc,\" and an abundant \"vitellogenin-like protein\"-were evaluated by western-blot analysis. A lateral flow immunoassay, using specific antibodies against the vitellogenin-like protein, successfully detected scabies ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Mar","modification":"2026-05-02T11:26:59.983Z","creation":"2025-04-05T17:16:16.766Z"},"accession":"S-EPMC7979781","cross_references":{"pubmed":["33742008"],"doi":["10.1038/s41598-021-85290-7"]}}