{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Qin Y"],"pubmed_abstract":["Polycystic ovary syndrome (PCOS) is a prevalent heterogeneous endocrine and metabolic disorder in women of reproductive age. Epigenetic mechanisms contribute to the development of PCOS. Nevertheless, the role of DNA methylation in the development of PCOS remains unclear. To investigate the molecular mechanisms underlying the hyperandrogenic phenotype of PCOS, dihydrotestosterone (DHT)-induced prenatally androgenized (PNA) mice were used to mimic this phenotype. Ovarian samples from PNA and control mice were subjected to methyl-CpG-binding domain (MBD)-seq and RNA-seq, and validation was conducted using methylation-specific polymerase chain reaction (MSP) and quantitative real-time PCR (RT-qPCR). Immunohistochemical analysis (using anti-LC3II antibody) and transmission electron microscopy w"],"journal":["Frontiers in genetics"],"pagination":["620241"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7982605"],"repository":["biostudies-literature"],"pubmed_title":["Integrated Transcriptomic and Epigenetic Study of PCOS: Impact of <i>Map3k1</i> and <i>Map1lc3a</i> Promoter Methylation on Autophagy."],"pmcid":["PMC7982605"],"pubmed_authors":["Li T","Ding C","Qin Y","Kang Y","Zhao H","Mao Z"],"additional_accession":[]},"is_claimable":false,"name":"Integrated Transcriptomic and Epigenetic Study of PCOS: Impact of <i>Map3k1</i> and <i>Map1lc3a</i> Promoter Methylation on Autophagy.","description":"Polycystic ovary syndrome (PCOS) is a prevalent heterogeneous endocrine and metabolic disorder in women of reproductive age. Epigenetic mechanisms contribute to the development of PCOS. Nevertheless, the role of DNA methylation in the development of PCOS remains unclear. To investigate the molecular mechanisms underlying the hyperandrogenic phenotype of PCOS, dihydrotestosterone (DHT)-induced prenatally androgenized (PNA) mice were used to mimic this phenotype. Ovarian samples from PNA and control mice were subjected to methyl-CpG-binding domain (MBD)-seq and RNA-seq, and validation was conducted using methylation-specific polymerase chain reaction (MSP) and quantitative real-time PCR (RT-qPCR). Immunohistochemical analysis (using anti-LC3II antibody) and transmission electron microscopy w","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2026-05-01T17:34:23.764Z","creation":"2024-11-21T10:22:57.417Z"},"accession":"S-EPMC7982605","cross_references":{"pubmed":["33763111"],"doi":["10.3389/fgene.2021.620241"]}}