{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Patten PEM"],"funding":["Medical Research Council","Blood Cancer UK"],"pagination":["627020"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7985329"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12"],"pubmed_abstract":["Patient-derived xenograft models of chronic lymphocytic leukemia (CLL) can be created using highly immunodeficient animals, allowing analysis of primary tumor cells in an <i>in vivo</i> setting. However, unlike many other tumors, CLL B lymphocytes do not reproducibly grow in xenografts without manipulation, proliferating only when there is concomitant expansion of T cells. Here we show that <i>in vitro</i> pre-activation of CLL-derived T lymphocytes allows for a reliable and robust system for primary CLL cell growth within a fully autologous system that uses small numbers of cells and does not require pre-conditioning. In this system, growth of normal T and leukemic B cells follows four distinct temporal phases, each with characteristic blood and tissue findings. Phase 1 constitutes a peri"],"journal":["Frontiers in immunology"],"pubmed_title":["A Detailed Analysis of Parameters Supporting the Engraftment and Growth of Chronic Lymphocytic Leukemia Cells in Immune-Deficient Mice."],"pmcid":["PMC7985329"],"funding_grant_id":["MR/T005106/1","09011","11039"],"pubmed_authors":["Patten PEM","Kolitz JE","Ferrer G","Allen SL","Ioannou N","Barrientos JC","Chen SS","Rai KR","Ramsay AG","Chiorazzi N"],"additional_accession":[]},"is_claimable":false,"name":"A Detailed Analysis of Parameters Supporting the Engraftment and Growth of Chronic Lymphocytic Leukemia Cells in Immune-Deficient Mice.","description":"Patient-derived xenograft models of chronic lymphocytic leukemia (CLL) can be created using highly immunodeficient animals, allowing analysis of primary tumor cells in an <i>in vivo</i> setting. However, unlike many other tumors, CLL B lymphocytes do not reproducibly grow in xenografts without manipulation, proliferating only when there is concomitant expansion of T cells. Here we show that <i>in vitro</i> pre-activation of CLL-derived T lymphocytes allows for a reliable and robust system for primary CLL cell growth within a fully autologous system that uses small numbers of cells and does not require pre-conditioning. In this system, growth of normal T and leukemic B cells follows four distinct temporal phases, each with characteristic blood and tissue findings. Phase 1 constitutes a peri","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2025-04-21T18:04:13.717Z","creation":"2024-11-09T10:48:17.859Z"},"accession":"S-EPMC7985329","cross_references":{"pubmed":["33767698"],"doi":["10.3389/fimmu.2021.627020"]}}